Edward G. Perkins

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Increasing the oleic acid (18:1 cis-9) content of milk fat might be desirable to meet consumer concerns about dietary healthfulness and for certain manufacturing applications. The extent to which milk fat could be enriched with oleic acid is not known. Increasing the intestinal supply of polyunsaturated fatty acids decreases dry matter intake (DMI) in cows,(More)
An HPLC method was developed using the C-30 carotenoid column to separate and identify the major xanthophylls in corn (lutein, zeaxanthin, and beta-cryptoxanthin). A photodiode array detector and a mobile phase consisting of methyl tert-butyl ether/methanol/water was used. All three xanthophylls eluted in less than 25 min. Yellow dent corn had a total(More)
Evidence for metabolic esterification of dehydroepiandrosterone (DHEA) in human blood plasma, identification of the active lipoprotein (LP) subclass involved, namely HDL3, as well as positive identification of the long-chain fatty acid esters of DHEA formed as incubation products is presented. The esterification reaction of DHEA and subsequent transfer and(More)
Incubation mixtures (1,2) contained the following components in a total volume of 0A ml: Tween 80. 100 #g; CF-54, 200 /zg; acceptor, 100 re#moles; UDP:(U14C) gal (International Chemical and Nuclear Corp.), 50 m/*moles containing 129,000 c.p.m.; particulate enzymes preparation from frog brains or rat brains, 0.7 mg of protein. After 4 hr at 25C, the mixtures(More)
Human milk triacylglycerols were separated by high-performance liquid chromatography. A 5-μ Supelcosil LC-18 column (Supelco, Inc., Bellefonte, PA) was used with acetone/acetonitrile (64∶36, vol/vol) as mobile phase. Triacylglycerols were tentatively identified based on theoretical carbon number and relative retention time. Despite changes resulting from(More)
The presence oftrans octadecenoic acid isomers was detected and analyzed in 24 hr pooled samples of human milk. Amounts of thetrans isomers of the C18 monoenes ranged from 2% to 4% of the total fatty acids. For purposes of comparison, three commonly used brands of infant formula were also analyzed and found to contain 0.1% to 1.3% oftrans monoene isomers.(More)
This study was conducted to investigate the effects of dietary cyclic fatty acid monomers (CFAM), contained in heated fat from a commercial deep-fat frying operation, on rat liver enzyme activity. A partially hydrogenated soybean oil (PHSBO) used 7 d (7-DH) for frying foodstuffs, or 0.15% methylated CFAM diets was fed to male weanling rats in comparison to(More)
This study reports the structural elucidation of diunsaturated 5- or 6-membered ring cyclic fatty acid monomers (CFAM) isolated from heated flaxseed oil by complementary gas chromatography (GC)-mass spectrometry (MS) and GC-matrix isolation-Fourier transform infrared spectroscopy (MI-FTIR). Infrared measurements of CFAM were carried out on methyl ester(More)