Eduardo Francisquine Delgado

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We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR(More)
Growth hormone treatment (GH) decreases adipose tissue sensitivity to insulin. However, the exact molecular mechanism(s) involved remains unclear. In the present study, we have evaluated the chronic effects of GH on adipose tissue explants cultured in a defined media. The objective was to determine the effects of GH treatment for 24 and 48 hours on the(More)
The Brazilian beef shows tenderness inconsistency. Moreover, most females slaughtered are cull cows, about six years which aggravates the lack of tenderness. There is the opportunity to mitigate this problem by meat ageing, but there is a question whether this would have impact on cows from Bos indicus genotype. The aim of this study was to evaluate the(More)
Feed regimens alter muscle growth rate, hence they might impact the proteolytic system involved in tenderization during meat conditioning. The aim of this project was to verify the effects of feed restriction regimens on muscular and animal growth and their impact on postmortem myofibrillar fragmentation. The regimens were: 1) Feeding ad libitum for 11 d(More)
Perception of color and its relationship to water holding capacity are important for defining the yield and quality of the pork production process. The aim of this study was to identify the relationship among color measurements taken at various anatomical positions in the cross-sectional surface of pork loin steak, and measurements of fluid exudation and(More)
Ninety-six feedlot-finished Nellore steers were used to verify the relationship between temperament indicators and meat tenderization. Temperament index (TI), chute score (CS), and average of exit velocity (EV) with CS (AT) presented low positive correlations (P≤0.05) with shear force, either at 2 or 16days postmortem (WBSF-16). The rectal temperature was(More)
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