Edna Nissani

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Intraerythrocytic malaria parasites produce vast amounts of lactic acid through glycolysis. While the egress of lactate is very rapid, the mode of extrusion of H+ is not known. The possible involvement of a Na+/H+ antiport in the extrusion of protons across the plasma membrane of Plasmodium falciparum has been investigated by using the fluorescent pH probe(More)
Quinoline-containing antimalarial drugs accumulate inside the acid food vacuole of the parasite where they inhibit the digestion of ingested host cell cytosol, and consequently, parasite growth. In order to verify whether this inhibition is caused by drug-induced alkalinization of the food vacuole, we investigated the accumulation of acridine orange (AO) as(More)
Esters of amino acids are known to penetrate into cells by simple diffusion. Subsequently, they are hydrolyzed by hydrolases to release the parent amino acid. Due to the abundance of hydrolases in phagolysosomes, amino acids accumulate, there because the rate of influx and hydrolysis exceed the rate of amino acid efflux through specific carriers. The(More)
The DNA of malarial parasites is significantly richer in A and T than that of mammalian cells. Antibiotics which bind to the minor groove of B-DNA with a preference for AT-rich sequences, such as distamycin A, netropsin, 4'-6-diamidino-2-phenylindole (DAPI) and bis-benzimide (Hoechst 33258) were found to inhibit the growth and propagation of Plasmodium(More)
Various lysosomotropic detergents were tested in this work on in vitro cultures of Plasmodium falciparum and are shown to be potent antimalarial agents. The order of antimalarial potency was similar to that of cell toxicity on mammalian cells in culture (Miller DK et al., J Cell Biol 97, 1841-51 (1983]. The most efficient agents, N-dodecyl-imidazole (NDI)(More)
Inside-out vesicles of Escherichia coli whose lumen was acidified by substrate oxidation, were used to study the mode of pH gradient dissipation by quinoline-containing antimalarial drugs and alkylamines. The pH was dissipated by micromolar drug concentrations, the dibasic chloroquine being most potent, followed by the monobasic mefloquine, quinine and the(More)
The binding of alizarin yellow G--an azo derivative of salicylic acid--by bovine serum albumin has been investigated using the method of equilibrium dialysis. Six strong and a number of additional, weak binding sites have been found to be present. The system is characterized by strong positive cooperativity between the first and second sites. Six binding(More)
The kinetics of the reaction between bilirubin and bovine serum albumin have been re-investigated at 20 degrees C. The results of previous authors concerning both human and bovine serum albumin were largely confirmed, namely the existence of two first-order configurational changes after a primary complex is formed in a fast, bimolecular step. From the(More)
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