Edivaldo Ximenes Ferreira Filho

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Hemicellulose is a complex group of heterogeneous polymers and represents one of the major sources of renewable organic matter. Mannan is one of the major constituent groups of hemicellulose in the wall of higher plants. It comprises linear or branched polymers derived from sugars such as d-mannose, d-galactose, and d-glucose. The principal component of(More)
Two endo-β-1,4-xylan xylanohydrolases (EC 3.2.1.8), XynA and XynB, from solid-state cultures ofPenicillium capsulatum, were purified to apparent homogeneity as judged by electrophoresis and isoelectric focusing. Each is a single subunit glycoprotein. XynA containing 97 mol carbohydrate·mol−1 protein, while XynB contains 63 mol·mol−1.M r and pI values are 28(More)
A new xylanase activity (XynII) was isolated from liquid state cultures of Acrophialophora nainiana containing birchwood xylan as carbon source. XynII was purified to apparent homogeneity by gel filtration and ion exchange chromatographies. The enzyme was optimally active at 55 degrees C and pH 7.0. XynII had molecular mass of 22630+/-3.0 and 22165 Da, as(More)
Xylan and mannan are the major constituent groups of hemicellulose in the cell wall of higher plants. The mesophilic fungus Trichoderma harzianum strain T4 produces extracellular xylanase and mannanase activities when grown in the presence of oat (Avena sativa)-spelt xylan and wheat bran as the carbon sources respectively. After the growth procedure, the(More)
A low-molecular-weight xylanase activity (XynI) was isolated from the fungus Acrophialophora nainiana after growth in a solid medium containing wheat bran. XynI was purified to apparent homogeneity by ultrafiltration and gel filtration chromatography. The purified enzyme had a molecular weight value of approx. 17 kDa, as determined by SDS-PAGE. This enzyme(More)
Nine Trichoderma harzianum strains were screened for β-xylosidase activity when grown in solid-state cultures on media containing wheat bran as the carbon source. All strains produced β-xylosidase activity, the most active being in extracts of cultures of T. harzianum strain 4. A β-xylosidase was purified by ammonium sulfate precipitation, ultrafiltration,(More)
Enzymes constitute a major monetary cost in the bioconversion of holocellulose to ethanol. Identifying enzyme inhibitors and moderating their effects is one approach that may help to overcome this issue. Most inhibitors that reduce the hydrolysis activity of holocellulases are released as the holocellulosic biomass is broken down in the pretreatment and(More)
Lignocellulose, the most abundant renewable carbon source on earth, is the logical candidate to replace fossil carbon as the major biofuel raw material. Nevertheless, the technologies needed to convert lignocellulose into soluble products that can then be utilized by the chemical or fuel industries face several challenges. Enzymatic hydrolysis is of major(More)
Two arabinofuranosidases, termed Ara I and Ara II, from solid-state cultures of Penicillium capsulatum were purified to apparent homogeneity as judged by electrophoresis and isoelectric focusing. Each enzyme is a single subunit glycoprotein, and they have M(r)s and pIs of 64,500 and 4.15 (Ara I) and 62,700 and 4.54 (Ara II), respectively. Ara I is most(More)