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Journals and Conferences
A simple method for estimation of the hydrophobic parameters (partition coefficient lg P and Hansch pi-value) of tetrazolium salts is reported using their relative velocities in reverse-phase layer chromatography. The experimental test values are close to relevant calculated values of the literature.
Succinate, malate, and lactate dehydrogenase were demonstrated histochemically and measured histophotometrically in the heart and skeletal muscle (m. extensor digitorum longus and m. soleus) of rats at different ages. To prove the value of histophotometry, the enzymes of the tissues were estimated biochemically. The gel film technique cannot sufficiently… (More)
During the incubation of cryostat sections (various organs of rat) with aqueous media xanthine dehydrogenase and superoxide dismutase activities become soluble and detectable in the solution by biochemical methods. Using the tetrazolium technique the demonstration of xanthine dehydrogenase is successful only after separation from the superoxide dismutase by… (More)
Modern results of the literature form the basis for the presentation of a scheme which shows the reduction of tetrazolium salts and the action of phenazine methosulfate under histochemical conditions. The reduction of tetrazolium salts passes the radical state and it hapens principally on the niveau of the flavin proteins and the ubiquinone. The importance… (More)
1. Transmitter glutamate/aspartate synthesis is known to proceed along different metabolic pathways. In this light, the functional relevance of glutamate dehydrogenase in postnatally maturing glutamatergic/aspartatergic structures was studied by means of quantitative enzyme histochemistry. 2. The basic requirements concerning the kinetics and calibration of… (More)
At present, tetrazolium salts are the most widely used indicators for the histochemical determination of dehydrogenases. The first salts that were used, for example, TTC and TV (triphenyl tetrazolium chloride and tetrazolium violet, respectively), were monotetrazolium compounds, but their histochemical properties such as precision of localization and… (More)
An account is given of latest knowledge on the structure of the human placenta. Reference is made to the early stages of development as well as to maturation under both normal and pathological conditions. The paper is intended to contribute to better understanding of the pathogenesis of chronic placental insufficiency.