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Two forms of α-glucan phosphorylase were isolated fromDioscorea cayenensis by ammonium sulphate gradient solubilization and further purified using starch adsorption and ion exchange chromatography on DEAE-Sephadex A-25 colunm. Fraction DC1was purified 80 fold with specific activity of 400 umol min−1 mg−1 protein, while fraction DC2showed 60 fold(More)
1. Superoxide dismutase activity has been determined in liver homogenates of five species. There were no significant differences in the activities of the enzyme in the rat, rainbow lizard, wall gecko and chicken. The activity was significantly lower in the fish. 2. The order of activity of the enzyme in the organs/tissues of the rat was liver greater than(More)
Phosphorylase from tubers ofDioscorea dumentorum was fractionated by (NH4)2SO4 precipitation and further purified to apparent homogeneity. Two forms of phosphorylase, Dd1 and Dd2 were obtained with different Km, optimum temperatures and pH values. They obeyed Michaelis-Menten kinetics, they were activated by AMP, Mg2+ and Ca2+ but inhibited by ATP, ADP,(More)
Waste disposal methods commonly practiced in Awka metropolis, Anambra state were investigated from August to October, 2013. Data was analyzed with both descriptive statistics of frequency and percentages, and alternate hypotheses were tested using Analysis of Variance (ANOVA) at a significance level of 0.05. Respondents in support of open burning were 61.2(More)
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