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Leafy Cotyledon Mutants of Arabidopsis.
The leafy cotyledon phenotype has allowed the identification of an important network of regulatory genes with overlapping functions during embryonic maturation in Arabidopsis, which indicates that immature embryos precociously enter a germination pathway after the torpedo stage of development and then acquire characteristics normally restricted to vegetative parts of the plant. Expand
Comprehensive developmental profiles of gene activity in regions and subregions of the Arabidopsis seed
This work profiled gene activity genome-wide in every organ, tissue, and cell type of Arabidopsis seeds from fertilization through maturity, offering the most comprehensive description of gene activity in seeds with high spatial and temporal resolution. Expand
The Accumulation of Oleosins Determines the Size of Seed Oilbodies in Arabidopsis[W][OA]
It is shown that oleosins are important proteins in seed tissue for controlling oilbody structure and lipid accumulation and their role in seed development or germination is investigated. Expand
Recent advances in conifer somatic embryogenesis: improving somatic embryo quality
The use of cell tracking techniques, implemented by physiological and molecular studies, has revealed that manipulations of the culture conditions early in the process can increase both number and quality of embryos produced in culture. Expand
Got milk? The secret life of laticifers.
The anatomy, development and physiology of laticifers are discussed with a focus on evolutionary and ecological perspectives. Expand
Embryogenesis in Angiosperms: Development of the Suspensor.
Although the suspensor appears to play a critical role in zygotic embryogenesis, it usually fails to develop when somatic embryos are produced in culture and should be viewed as a specialized structure that functions primarily to facilitate continued development of the embryo proper within the seed. Expand
Disruption of morphogenesis and transformation of the suspensor in abnormal suspensor mutants of Arabidopsis
This report focuses on seven abnormal suspensor mutants of Arabidopsis that exhibit an enlarged suspensor phenotype at maturity, which is consistent with a model in which normal development of the embryo proper limits growth and differentiation of the suspensor. Expand
The use of histology in the study of plant tissue culture systems—Some practical comments
  • E. Yeung
  • Biology
  • In Vitro Cellular & Developmental Biology - Plant
  • 1 March 1999
This article serves to draw attention to the use of a histological approach to one’s experimental system by discussing some of the common mistakes in the handling and processing of explants. Expand
An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture
High-frequency protocorm-like body (PLB) formation directly from thin leaf sections of Doritaenopsis hybrid was achieved in order to develop a mass-scale propagation system and light microscopy observations indicated that the initial cell divisions for PLB formation occurred on the region near the cut surface and that an intact epidermal layer appeared to play an important role inPLB formation. Expand
Structural and Developmental Patterns in Somatic Embryogenesis
This chapter provides a synthesis of the information available concerning the structural and developmental patterns observed during somatic embryogenesis, and selected, well-characterized systems will be discussed. Expand