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Functional profiling of the Saccharomyces cerevisiae genome
It is shown that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment, and less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal Growth in four of the tested conditions.
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome), finding that 17 percent were essential for viability in rich medium.
A genome-wide transcriptional analysis of the mitotic cell cycle.
Replication dynamics of the yeast genome.
Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae, finding the two ends of each of the 16 chromosomes are highly correlated in their times of replication.
Discovery of Gene Function by Expression Profiling of the Malaria Parasite Life Cycle
A high-density oligonucleotide array is used to generate expression profiles of human and mosquito stages of the malaria parasite's life cycle and finds genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes.
Large-scale identification of single-feature polymorphisms in complex genomes.
A high-throughput genotyping platform is developed by hybridizing genomic DNA from Arabidopsis thaliana accessions to an RNA expression GeneChip (AtGenome1), and it is demonstrated that array hybridization can be combined with bulk segregant analysis to quickly map mutations.
Genomics, gene expression and DNA arrays
Measurements of gene expression and other applications of arrays embody much of what is implied by the term ‘genomics’; they are broad in scope, large in scale, and take advantage of all available sequence information for experimental design and data interpretation in pursuit of biological understanding.
The core meiotic transcriptome in budding yeasts
High-density oligonucleotide microarrays were used to analyse the genomes and meiotic expression patterns of two yeast strains that display distinct kinetics and efficiencies of sporulation and yielded approximately 1,600 meiotically regulated genes in each strain.
Spiroindolones, a Potent Compound Class for the Treatment of Malaria
The preclinical profile for an optimized spiroindolone drug candidate, NITD609, shows pharmacokinetic properties compatible with once-daily oral dosing and has single-dose efficacy in a rodent malaria model.