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PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery
We investigated the in vitro and in vivo properties of DNA/transferrin–polyethylenimine (800 kDa) complexes before and after covalent coupling of poly(ethylene glycol) (PEG). Upon incubation withExpand
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Design and gene delivery activity of modified polyethylenimines.
The polycation polyethylenimine (PEI) has recently been widely employed for the design of DNA delivery vehicles. Gene delivery using PEI involves condensation of DNA into compact particles, uptakeExpand
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Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo
Efficient gene transfer is a major challenge for non‐viral gene therapy. Understanding how non‐viral vectors initiate gene expression could lead to the development of new future vectors with enhancedExpand
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The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems.
The process by which viruses destabilize endosomal membranes in an acidification-dependent manner has been mimicked with synthetic peptides that are able to disrupt liposomes, erythrocytes, orExpand
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  • Open Access
Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer
Nonviral vectors based on polyethylenimine (PEI) usually contain an excess of PEI that is not complexed to DNA. Since unbound PEI contributes to cellular and systemic toxicity, purification ofExpand
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Apoptosis-Inducing Factor Triggered by Poly(ADP-Ribose) Polymerase and Bid Mediates Neuronal Cell Death after Oxygen-Glucose Deprivation and Focal Cerebral Ischemia
Delayed neuronal cell death occurring hours after reperfusion is a hallmark of ischemic stroke and a primary target for neuroprotective strategies. In the present study, we investigated whetherExpand
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  • Open Access
Novel shielded transferrin-polyethylene glycol-polyethylenimine/DNA complexes for systemic tumor-targeted gene transfer.
Tumor-targeting DNA complexes which can readily be generated by the mixing of stable components and freeze-thawed would be very advantageous for their subsequent application as medical products.Expand
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Activation of the complement system by synthetic DNA complexes: a potential barrier for intravenous gene delivery.
We have examined the complement-activating properties of synthetic cationic molecules and their complexes with DNA. Commonly used gene delivery vehicles include complexes of DNA with polylysine ofExpand
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Mannose Polyethylenimine Conjugates for Targeted DNA Delivery into Dendritic Cells*
Cell surface-bound receptors represent suitable entry sites for gene delivery into cells by receptor-mediated endocytosis. Here we have taken advantage of the mannose receptor that is highlyExpand
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  • Open Access
Angiogenesis in the mouse lung.
When pulmonary arterial blood flow is obstructed in all mammals studied, there is a compensatory growth of the bronchial vasculature. This angiogenesis normally occurs through a proliferation of theExpand
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