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Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA
TLDR
The DGGE analysis of environmental samples collected from Norway spruce stumps showed that the analysis of fungal DNA extracted directly from wood was usually in accordance with the investigation of mycelial cultures isolated from the same decay column, however, disagreement was observed, which suggests that these two fundamentally different techniques present different views about fungal diversity.
Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research.
TLDR
The focus of this review is the taxonomic reorganization of family Partitiviridae (encapsidated, bisegmented dsRNA viruses that infect plants, fungi, or protozoa), as well as redistribution of species among these new genera.
Genetic variation in Phlebiopsis gigantea as detected with random amplified microsatellite (RAMS) markers
TLDR
The high degree of polymorphism and heterozygosity observed in RAMS markers suggests that the amount of genetic variation within this fungus is considerable and supports the idea of P. gigantea being a true biological species consisting of a single intersterility group.
Investigations on Heterobasidion annosum s.lat. in central and eastern Asia with the aid of mating tests and DNA fingerprinting
TLDR
To investigate the taxonomy of Heterobasidion in Eurasia, 49 specimens belonging to H. annosum sensu lato from Asia were identified with the aid of mating tests and showed a high degree of compatibility with H. parviporum and H. abietinum.
Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs.
TLDR
Quantification of viral double- and single-stranded RNA with quantitative PCR indicated that co-infecting viral species and viruses with segmented genomes can be detected with small RNA deep sequencing despite vast differences in the amount of RNA.
IgA ANTIGLIADIN ANTIBODIES: A MARKER OF MUCOSAL DAMAGE IN CHILDHOOD COELIAC DISEASE
TLDR
Rising IgG antigliadin antibody levels after gluten elimination were seen in 6 patients, 5 of whom had very low complement C3 levels before gluten elimination, and in 2 IgA-deficient patients gluten challenge caused an increase in IgM antIGliadin antibodies, and at the same time the number of IgM-containing cells increased in the jejunal mucosa.
Characterization of Cyanobacteria by SDS-PAGE of whole-cell proteins and PCR/RFLP of the 16S rRNA gene
TLDR
Planktonic, filamentous cyanobacterial strains from different genera, both toxic and nontoxic strains, were characterized by SDS-PAGE of whole-cell proteins and PCR/RFLP of the 16S rRNA gene, confirming that close genotypic relationships underlie considerable diversity among cyanob bacterial genera.
A novel putative partitivirus of the saprotrophic fungus Heterobasidion ecrustosum infects pathogenic species of the Heterobasidion annosum complex.
TLDR
The bisegmented genome of a putative double-stranded RNA virus from a Chinese isolate of the fungus H. ecrustosum, a member of the Heterobasidion insulare species complex, was characterized and suggested a possible route for horizontal transmission between these sexually incompatible species.
Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidion annosum sensu lato.
TLDR
A novel putative mycovirus infecting the conifer root-rot fungus Heterobasidion annosum sensu lato showed a considerable degree of geographical and host-related differentiation, and seemed to be cryptic or slightly mutualistic to its host.
Circulating IgA- and IgG-class antigliadin antibodies in dermatitis herpetiformis detected by enzyme-linked immunosorbent assay
TLDR
The occurrence of circulating IgA-class AGA is compatible with the hypothesis that these antibodies can be deposited in the skin, e.g. as immune complexes, or due to cross-reactivity of gliadin and dermal reticulin.
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