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Isolation, and catalytic and immunochemical properties of cathepsin D-like acid proteinase from rat erythrocytes.
The results indicate that EMAP is a unique cathepsin D-like acid proteinase different from ordinary cathepped D, which was purified to homogeneity from freshly collected rat blood in a yield of 60-65%. Expand
Activation mechanism of erythrocyte cathepsin E. evidence for the occurrence of the membrane-associated active enzyme.
The present study provides the first evidence that cathepsin E tightly bound to the membrane is converted to the active enzyme in the membrane-associated form, and suggests that this enzyme may be responsible for the degradation of band 3. Expand
Evaluation of dot-immunobinding assay for carcinoembryonic antigen determination in nipple discharge as an adjunct in the diagnosis of early breast cancer. Research Group for Carcinoembryonic Antigen
A dot-immunobinding assay was developed to screen a large number of patients with nipple discharge for non-palpable breast cancer and found the system could prove useful in screening for early breast cancer. Expand
Expression of growth factors and their receptors in human early colorectal carcinomas: immunohistochemical study.
Results indicate that two pathways exist in tumourigenesis, in which the growth factors and their receptors are expressed in different manners, and TGF-alpha might play a crucial role in carcinomas arising from adenoma, while EGF and c-ERBB2 gene products are strongly indicative of de novo carcinomas. Expand
Stabilisation of cathepsin E by ATP
At still higher pH values, k cat progressively diminished but significant levels of cathepsin E activity were readily detectable at pH 7.0, and the specificity of this stabilisation effect was examined. Expand
Biochemical and immunochemical similarity between erythrocyte membrane aspartic proteinase and cathepsin E.
An aspartic proteinase previously thought to be unique to erythrocyte membranes, termed "EMAP", has been shown to be closely related to cathepsin E, which resembled each other in molecular weight, susceptibility to pepstatin and chromatographic behaviors on DEAE-Sephacel and Mono P chromatofocusing columns. Expand
Reactivity of recombinant treponema pallidum (r‐Tp) antigens with anti‐Tp antibodies in human syphilitic sera evaluated by ELISA
G17 antigen was more effective in detecting syphilis antibodies in human serum than M47, S42, and G15, and there was a good consistency between ELISA and TPHA, whereby the cutoff indexes (CI) on ELISA showed a correlation coefficient of 0.7276 in logarithmic TPHA titers. Expand
Biological significance and activity control of cathepsin E compared with cathepsin D.
Protein degradation in mammalian cells is thought to occur via two major pathways: a lysosomal and a non-lysosomal pathway. The former may participate in degradation of the majority of cellularExpand
Antisense oligonucleotide inhibits the expression of procollagen type III gene in mouse fibroblasts.
Transfection of phosphorothioate antisense oligonucleotides into BALB/c 3T3 mouse fibroblasts showed ASOs were effective for inhibiting procollagen type III production at the gene level, although the effect was transient. Expand
Establishment of monoclonal antibody, gp21-34, against HTLV-II envelope protein (p20E).
A monoclonal antibody (MAb), gp21-34, specifically reactive with human T-lymphotropic virus type-II (HTLV-II) transmembranous envelope glycoprotein (p20E) was developed by immunization with aExpand