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Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi
The sulfated galactofucan was 2-fold more potent than heparin in stimulating the synthesis of an antithrombotic heparan sulfate by endothelial cells, and suggested that this last effect may be related to the “in vivo” antithROMbotic activity of this polysaccharide. Expand
Freshwater Plants Synthesize Sulfated Polysaccharides: Heterogalactans from Water Hyacinth (Eicchornia crassipes)
Eichornia SP demonstrated promising anticoagulant potential and have been selected for further studies on bioguided fractionation; isolation and characterization of pure polysaccharides from this species. Expand
Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells.
It is suggested that the antithrombotic activity of heparin and other antithromabotic agents is due at least in part to their action on endothelial cells stimulating the synthesis of an antithROMbotic heparan sulfate. Expand
In vivo and in vitro cytotoxicity of brown spider venom for blood vessel endothelial cells.
The above results suggest that the L. intermedia venom has a deleterious effect on the endothelium of vessels both in vivo and in culture, removing important constituents such as HSPG and entactin that are involved in the adhesion of endothelial cells and of subendothelial ECM organization. Expand
Development of an enzyme-linked immunosorbent assay (ELISA)-like fluorescence assay to investigate the interactions of glycosaminoglycans to cells.
A convenient and reliable method for the preparation of biotinylated glycosaminoglycans, as well as a sensitive non-competitive fluorescence-based assay for studies of the interactions and binding of these compounds to cells in culture are proposed. Expand
Fucan effect on CHO cell proliferation and migration.
Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation, which inhibits CHO-K1 migration on fibronECTin and ERK blocks cell cycle progression. Expand
Sulfation of the extracellular polysaccharide produced by the edible mushroom Pleurotus sajor-caju alters its antioxidant, anticoagulant and antiproliferative properties in vitro
Abstract An extracellular polysaccharide (PN) composed of mannose:3-O-methyl-galactose:galactose:glucose (44.9:16.3:19.8:19) was obtained from Pleurotus sajor-caju . This polymer showed specificExpand
Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes: Cestoda).
It is demonstrated that both praziquantel and albendazole cause extensive tissue damage, especially on tegument, and that adult forms were the most susceptible to drug exposure. Expand
Effects of anti-inflammatory drugs in primary kidney cell culture of a freshwater fish.
The pharmaceuticals influenced NO production and caused DNA damage in monocytic cells suggesting that these drugs can induce immunosuppression and genotoxicity in fish. Expand
The binding of heparin to the extracellular matrix of endothelial cells up‐regulates the synthesis of an antithrombotic heparan sulfate proteoglycan
The results indicate that the stimulus for the synthesis of HS does not require binding of the heparin to the cell surface, and the signaling may be mediated through the ECM. Expand