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Microbial production, characterization and applications of feruloyl esterases
FAEs act synergistically with xylanases to hydrolyze ester-linked ferulic and diferulic acid from cell wall material and therefore play a major role in the degradation of plant biomass.
Hydrolysis and fermentation of brewer's spent grain by Neurospora crassa.
Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to characterize the major activities of the multienzyme component, after the separation by isoelectric focusing (IEF) electrophoresis.
Performance of an intermittent agitation rotating drum type bioreactor for solid-state fermentation of wheat straw.
The suitability of the apparatus for the effective control of important operating variables affecting growth of microbes in solid-state cultivation was determined and the correlation between microorganism growth and production of enzymes was efficiently described by the Le Duy kinetic model.
Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis
Abstract A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M r 23,000 and pI 3.1. The enzyme activity
Purification and characterization of a type B feruloyl esterase (StFAE-A) from the thermophilic fungus Sporotrichum thermophile
The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and it hydrolyzed 4-nitrophenyl 5-O-trans-feruloyl-α-l-arabinofuranoside 2-fold more efficiently than NPh-2-Fe-Araf.
The Cellvibrio japonicus Mannanase CjMan26C Displays a Unique exo-Mode of Action That Is Conferred by Subtle Changes to the Distal Region of the Active Site*
How subtle differences in the loops surrounding the active site of a glycoside hydrolase can lead to a change in the mode of action of the enzyme is revealed.
The feruloyl esterase system of Talaromyces stipitatus: determining the hydrolytic and synthetic specificity of TsFaeC.
The active site of the recombinant Talaromyces stipitatus type-C feruloyl esterase (TsFaeC) was probed using a series of C1-C4 alkyl ferulates and methyl esters of phenylalkanoic and cinnamic acids to demonstrate the importance of hydroxyl groups on meta and/or para position of the benzoic ring.
Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile.
The synergistic activity exhibited by StCel61a and its dependence on reducing substances provide guidelines for process design towards the production of economically viable bioethanol.
Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures.
Determination of k(cat)/K(m) revealed that the enzyme hydrolysed methyl sinapinate 6, 21 and 40 times more efficiently than methyl ferulate, methyl coumarate and methyl caffeate, respectively, indicating a synergistic interaction between FAE-II and S. thermophile xylanase.
Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum
The putative FAE from the genomic DNA was successfully cloned in frame with the Saccharomyces cerevisiae α-factor secretion signal under the transcriptional control of the alcohol oxidase (AOX1) promoter and integrated in Pichia pastoris X-33 to confirm that the enzyme exhibits FAE activity.