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Somatic embryogenesis from immature embryos of redbud (Cercis canadensis)
Although somatic embryogenesis was expressed on most media, the number of explants that produced somatic embryos and the mean number of embryos formed per explant were greatest on media that contained either 2 or 3 mg/1 2,4-D; 12.6 mM ammonium ion inhibited embryogenesis from 96 day post-anthesis explants.
Plant cuticle staining with bismarck brown Y and azure B or toluidine blue O before paraffin extraction.
The interface between the cuticle and exterior cell walls of the epidermis was delineated clearly and the Bismarck brown was replaced by thiazin dye metachromasia in all structures except the cuticles which remained brown or yellow.
The Association of Discula destructiva (Red.) Hyphae With Cornus florida (L.) Trichomes
Scanning electron micrographs show an association of D. destructiva hyphae with trichomes on the flowering dogwood upper leaf surface, which may provide an entry point for Discula spp.
Nitrogen source and calcium supplement affect stem strength of poinsettia
Stem retention was increased in the AHDR and C-1 that received Ca(No 3 ) 2 liquid fertilization and the effects of nitrate and NH 4 NO 3 used alone or in combinations with CaCl 2 sprays were examined.
Improved diethylene glycol distearate embedding wax.
- E. T. Graham
- ChemistryStain technology
Diethylene glycol distearate wax and cellulose caprate resin, 4:1 respectively by weight, were melted together at 75 C for five hours with occasional stirring, and formed flat sections more consistently than the pure wax.
Ontogeny and ultrastructure of spontaneous nodules in alfalfa (Medicago sativa)
The results suggest that bacteria and therefore theirnod genes are not necessarily involved in the ontogeny and morphogenesis of spontaneous and normal nodules in alfalfa.
A histological study of tissue proliferation, embryogenesis, and organogenesis from tissue cultures ofDactylis glomerata L.
Histological information is presented on the origin of initial tissue proliferation and on embryogenesis and organogenesis in sub-cultured tissue derived from mature orchardgrass embryos and on somatic embryos completely isolated from the tissue mass suggestingde novo embryogenesis.
Staining paraffin extracted, alcohol rinsed and air dried plant tissue with an aqueous mixture of three dyes.
A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer and structures could be distinguished within 30 min as follows: nonlignified cell walls, blue; lignifiedcell walls, nuclei and chloroplasts, red; and cuticle, brown or yellow-brown.
Novel fixation of plant tissue, staining through paraffin with alcian blue and hematoxylin, and improved slide preparation.
The traditional paraffin microtechnique has been modified at all steps from fixation to finishing slides with coverslips, especially for onion root tips fixed in a proprietary solution without aldehyde, toxic metals or acetic acid.
Ultrastructure of transfer cells in spontaneous nodules of alfalfa (Medicago sativa)
- P. A. Joshil, G. Caetano-Anollés, E. T. Graham, P. Gresshoff
- Environmental ScienceProtoplasma
- 1 June 1993
The results suggest that xylem parenchyma and phloem companion transfer cells are active and probably involved in the short distance transport of solutes in and out of spontaneous nodules, and fusion of ER-originated vesicles with wall ingrowth membrane indicated the involvement of ER in the membrane formation for elongating wall ingrowths.