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Determination of carbonyl content in oxidatively modified proteins.
TLDR
This chapter discusses methods to determine carbonyl content in oxidatively modified proteins and quantitated protein-bound pyruvoyl groups through formation of a Schiff base with p-aminobenzoic acid followed by reduction with cyanoborohydride. Expand
Protein Oxidation in Aging, Disease, and Oxidative Stress*
Carbonyl assays for determination of oxidatively modified proteins.
TLDR
New methods for determination ofcarbonyl content are presented, which are based on the reaction of carbonyl groups with 2,4-dinitrophenylhydrazine to form a 2, 4-d Initrophenolhydrazone, which provide substantial improvements in both sensitivity and specificity. Expand
Free radical-mediated oxidation of free amino acids and amino acid residues in proteins
TLDR
It is evident that the cyclic oxidation and reduction of the sulfur-containing amino acids may serve as an important antioxidant mechanism, and also that these reversible oxidations may provide an important mechanism for the regulation of some enzyme functions. Expand
Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors.
TLDR
It is shown that H2O2 produced in response to stimulation of cells with epidermal growth factor or platelet-derived growth factor potentiates PIP3 generation and activation of the protein kinase Akt induced by these growth factors, and that peroxiredoxin likely participates in PIP2 signaling by modulating the local concentration of H2 O2. Expand
Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions.
  • E. Stadtman
  • Chemistry, Medicine
  • Annual review of biochemistry
  • 1993
TLDR
Results of these studies indicate that the most common pathway for the oxidation of simple aliphatic amino acids involves the hydroxyl radical-mediated abstraction of a hydrogen atom to form a carbon-centered radical at the alpha-position of the amino acid or amino acid residue in the polypeptide chain. Expand
Methionine residues as endogenous antioxidants in proteins.
TLDR
This work proposes that methionine residues constitute an important antioxidant defense mechanism, and investigates the effect of hydrogen peroxide exposure upon glutamine synthetase from Escherichia coli as an in vitro model system. Expand
Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease.
TLDR
It is concluded that protein oxidation products accumulate in the brain and that oxidation-vulnerable enzyme activities decrease with aging in the same regional pattern (frontal more affected than occipital) and that AD may represent a specific brain vulnerability to age-related oxidation. Expand
Protein oxidation and aging
TLDR
The importance of protein oxidation in aging is supported by the observation that levels of oxidized proteins increase with animal age and may reflect age-related increases in rates of ROS generation, decreases in antioxidant activities, or losses in the capacity to degrade oxidized protein. Expand
Protein oxidation and aging.
  • E. Stadtman
  • Chemistry, Medicine
  • Free radical research
  • 28 August 1992
TLDR
The importance of protein oxidation in aging is supported by the observation that levels of oxidized proteins increase with animal age and may reflect age-related increases in rates of ROS generation, decreases in antioxidant activities, or losses in the capacity to degrade oxidized protein. Expand
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