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Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival.
Cryopreservation induces partially irreversible damage to equine sperm membranes. Part of this damage occurs due to membrane alterations induced by the membrane changing from the fluid to the… Expand
Embryo technologies in the horse.
Recent studies demonstrated that zwitterionic buffers could be used for satisfactory storage of equine embryos at 5 degrees C. The success of freezing embryos is dependent upon size and stage of… Expand
Evaluation of alternative cryoprotectants for preserving stallion spermatozoa.
Although use of cryopreserved stallion spermatozoa is currently accepted by many breed registries, utilization of this technique remains limited due to poor fertility for some stallions. One reason… Expand
Factors affecting pregnancy rates and early embryonic death after equine embryo transfer.
- E. Carnevale, R. J. Ramirez, E. Squires, M. Alvarenga, D. K. Vanderwall, P. Mccue
- Biology, Medicine
- 1 October 2000
In the present study, 638 embryo transfers conducted over 3 yr were retrospectively examined to determine which factors (recipient, embryo and transfer) significantly influenced pregnancy and embryo… Expand
The current status of equine embryo transfer.
The use of embryo transfer in the horse has increased steadily over the past two decades. However, several unique biological features as well as technical problems have limited its widespread use in… Expand
Seasonal variation in hypothalamic content of gonadotropin-releasing hormone (GnRH), pituitary receptors for GnRH, and pituitary content of luteinizing hormone and follicle-stimulating hormone in the…
Seasonal changes in the hypothalamic-hypophyseal axis were investigated using tissue from 49 light-horse mares, of mixed breeding. Hypothalamic and pituitary tissues were collected at 5 intervals… Expand
Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos.
- W. D. Eldridge-Panuska, V. C. di Brienza, G. Seidel, E. Squires, E. Carnevale
- Biology, Medicine
- 15 March 2005
Experiments were conducted to determine viability of equine embryos in vivo after vitrification. In a preliminary study (Experiment 1), embryos were exposed in three steps to vitrification solutions… Expand
Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage.
The objective of this study was to determine if centrifugation and partial removal of seminal plasma would improve spermatozoal motility in semen from stallions whose whole ejaculates have poor… Expand
Factors affecting motion characteristics of frozen-thawed stallion spermatozoa.
- A. Heitland, D. Jasko, E. Squires, J. K. Graham, B. W. Pickett, C. Hamilton
- Equine veterinary journal
Five experiments were conducted to evaluate damage incurred in each processing step for cryopreservation of stallion spermatozoa. In Experiment 1, semen was centrifuged for 9 centrifugation times and… Expand
Effect of seminal plasma and egg yolk on motion characteristics of cooled stallion spermatozoa
Abstract This study on extended, cooled spermatozoa was designed to determine 1) the concentration of seminal plasma required for the preservation of sperm motility, 2) whether the addition of egg… Expand