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High performance chromatographic separation of inositol phosphate isomers on strong anion exchange columns.
Inositol phosphates are focused on because of their functions in the cell, as well as their effects on the bioavailability of certain minerals. To improve the separation of inositol hexakis- to
Inositol phosphates with different numbers of phosphate groups influence iron absorption in humans.
To improve iron absorption from cereals and legumes, degradation of inositol phosphates needs to be to less-phosphorylated inositoli phosphates than IP(3), while IP(5) has an inhibitory effect on iron absorption, whereas IP (3) and IP(4) in isolated form have no such effect.
Determination of Isomers of Inositol Mono- to Hexaphosphates in Selected Foods and Intestinal Contents Using High-Performance Ion Chromatography
A sensitive high-performance ion chromatography (HPIC) method for separation and quantitative determination of inositol mono- to hexaphosphate (IP1−IP6) isomers in selected foods and intestinal
Rapid analysis of inositol phosphates.
The between- and within-day variances were generally below 8 and 5% (relative standard deviation), respectively, for the presented HPIC method with gradient elution.
Phytate hydrolysis in pigs fed a barley-rapeseed meal diet treated with Aspergillus niger phytase or steeped with whey.
The degradation of phytate (myo-inositol hexaphosphate) in a barley-rapeseed meal (80:20) diet due to supplemented Aspergillus niger phytase and steeping (soaking at 40 °C for 3 h with feed to water
Comparison between steeping and pelleting a mixed diet at different calcium levels on phytate degradation in pigs.
The degradation of phytate in the stomach, small intestine and colon was studied in 36 female pigs and apparent phosphorus absorption from ileal digesta was 10% lower when pigs were fed the pelleted diet, as compared to the steeped diet.
Combined approach for high-throughput preparation and analysis of plasma samples from exposure studies.
A combined approach is described for the high-throughput preparation and liquid chromatography/tandem mass spectrometry analysis of drug levels in plasma samples from the preclinical efficacy and safety studies, i.e. exposure studies, based on the combined use of PK simulations, a liquid handling system and a fast LC/MS/MS method.
Analysis of Inositol Mono- and Diphosphate Isomers Using High-Performance Ion Chromatography and Pulsed Amperometric Detection
A rapid and sensitive high-performance ion chromatography method for separation and quantitative determination of inositol mono- and diphosphate (IP1−IP2) isomers using pulsed amperometric detection
Description and validation of a four-channel staggered LC-MS/MS system for high-throughput in vitro screens.
A parallel four-channel LC-MS/MS system for quantitative high-throughput in vitro screens is described, and the results showed that the sample throughput could be increased more than three times in comparison with a conventional single-column LC- MS/ MS system.
Inositol phosphates influence iron uptake in Caco-2 cells.
Iron uptake was shown to not be a function of the isomeric form of inositol phosphates, and the inositl phosphate isomers did not seem likely to interact with each other through iron to form more stable iron complexes.