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E6 – PHYLOGENETIC CLASSIFICATION OF PLANT PATHOGENIC MYCOPLASMA-LIKE ORGANISMS OR PHYTOPLASMAS
This chapter presents a phytoplasma taxonomic scheme that is based on the analysis of two evolutionary markers: the 16S ribosomal ribonucleic acid (rRNA) gene and the spacer region that separates theExpand
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Current status of molecular classification of the phytoplasmas.
Phytoplasmas for which 16S rDNA sequences are available have been classified into 20 major phylogenetic groups or subclades. Further phytoplasmas have been assigned to these groups, according toExpand
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'Candidatus Phytoplasma asteris', a novel phytoplasma taxon associated with aster yellows and related diseases.
Aster yellows (AY) group (16SrI) phytoplasmas are associated with over 100 economically important diseases worldwide and represent the most diverse and widespread phytoplasma group. Strains thatExpand
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Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene.
A polymerase chain reaction (PCR) method has been developed with a 558-bp fragment of the 16S rRNA gene as template DNA and two oligonucleotide primers from conserved regions of this gene. TheExpand
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Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA.
Ribosomal and nonribosomal sequences were analyzed to design polymerase chain reaction (PCR) primers for detection and identification of the phytoplasmas that cause apple proliferation (AP) and pearExpand
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'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma pyri' and 'Candidatus Phytoplasma prunorum', the causal agents of apple proliferation, pear decline and European stone fruit yellows,
Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analysesExpand
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Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure
A polymerase chain reaction procedure was developed which enables specific amplification of a ribosomal sequence from the mycoplasmalike organism (MLO) associated with German grapevine yellowsExpand
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Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA.
The phylogenetic relationships of 17 phytopathogenic mycoplasmalike organisms (MLOs) representing seven major taxonomic groups established on the basis of MLO 16S ribosomal DNA (rDNA) restrictionExpand
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Classification of aster yellows-group phytoplasmas based on combined analyses of rRNA and tuf gene sequences.
Seventy phytoplasma isolates, including 10 previously characterized reference strains, of the aster yellows group were examined by RFLP analysis of PCR-amplified rDNA and RFLP and sequence analysisExpand
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