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Amplification and disruption of the phenylacetyl-CoA ligase gene of Penicillium chrysogenum encoding an aryl-capping enzyme that supplies phenylacetic acid to the isopenicillin N-acyltransferase.
A gene, phl, encoding a phenylacetyl-CoA ligase was cloned from a phage library of Penicillium chrysogenum AS-P-78. The presence of five introns in the phl gene was confirmed by reverseExpand
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Coupling solid-phase microextraction and high-performance liquid chromatography for direct and sensitive determination of halogenated fungicides in wine.
A solid-phase microextraction (SPME) method coupled to high-performance liquid chromatography with diode array detection (HPLC-DAD) for the analysis of six organochlorine fungicides (nuarimol,Expand
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Determination of diflubenzuron and its main metabolites in forestry matrices by liquid chromatography with on-line diode-array and electrochemical detection
Abstract A liquid chromatography (LC) method with on-line diode-array (DAD) and electrochemical detection (ED) has been developed for the determination of the pesticide diflubenzuron (DFB) and itsExpand
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A quantitative FastFUCCI assay defines cell cycle dynamics at a single-cell level
ABSTRACT The fluorescence ubiquitination-based cell cycle indicator (FUCCI) is a powerful tool for use in live cells but current FUCCI-based assays have limited throughput in terms of imageExpand
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The disposition of free and niosomally encapsulated Rac-flurbiprofen in dairy bovines.
Pharmacokinetic parameters were established for flurbiprofen (FBP) after intravenous (i.v.) administration (0.5 mg/kg) of niosomal and nonniosomal formulations in dairy cattle. Niosomes of FBP showedExpand
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Photo-Assisted Oxidation of Indigocarmine in an Acid Medium
The kinetics of photo-oxidation of indigocarmine dye—5,5-indigosulfonate disodium (5,5-IDS)—in an acid medium has been studied to discover the effect of different parameters in the efficiency of theExpand
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Multisorbent tubes sampling used in thermal desorption cold trap injection with gas chromatography-mass spectrometry for C2–C6 hydrocarbon measurements in an urban atmosphere
Three solid adsorbents (Carbotrap, Carbotrap C, and Carbosieve III) were evaluated for sampling trace non-methane hydrocarbons in urban atmospheres. The sampled atmosphere was pumped through aExpand
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Determination of glutathione, cysteine and N-acetylcysteine in rabbit eye tissues using high-performance liquid chromatography and post-column derivatization with 5,5'-dithiobis(2-nitrobenzoic acid).
A high-performance liquid chromatographic method to determine glutathione, cysteine and N-acetylcysteine in rabbit retina, vitreous and lens has been developed. The thiols are separated using a 25 xExpand
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Determination of catecholamines and their metabolites in human plasma using liquid chromatography with coulometric multi-electrode cell-design detection
Abstract A solid-phase extraction method (SPE) combined with liquid chromatography with coulometric multi-electrode detection for the analysis of the main endogenous catecholamines and itsExpand
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Quantification of fenitrothion and its main metabolites in poplar leaves by isotope dilution gas chromatography-mass spectrometry coupled with solid-phase microextraction.
A sensitive and specific method for determining fenitrothion and its main metabolites, 3-methyl-4-nitrophenol and fenitrooxon, in poplar leaves using deuterated isotopes as the internal standard isExpand
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