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Specific Substitutions in the Echinocandin Target Fks1p Account for Reduced Susceptibility of Rare Laboratory and Clinical Candida sp. Isolates
TLDR
Results indicate that substitutions in the Fks1p subunit of GS are sufficient to confer reduced susceptibility to echinocandins in S. cerevisiae and the pathogens C. albicans and C. krusei.
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An improved protocol for the preparation of yeast cells for transformation by electroporation
TLDR
Pretreatment of yeast cells with lithium acetate and dithiothreitol enhances the frequency of transformation by electroporation and results in 15–300‐fold improvement in transformation frequency with several mutant strains of S. cerevisiae that transformed poorly by conventional procedures.
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Isolation of a gene involved in 1,3-beta-glucan synthesis in Aspergillus nidulans and purification of the corresponding protein
TLDR
The product of fksA is important for the activity of highly purified preparations of GS, either as the catalytic subunit itself or as an associated copurifying subunit that mediates susceptibility of enzymatic activity to echinocandin inhibition.
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Geranylgeranyltransferase I of Candida albicans: Null Mutants or Enzyme Inhibitors Produce Unexpected Phenotypes
TLDR
It is hypothesized that Cdc42p and Rho1p of C. albicans can be prenylated by FTase when GGTase I is absent or limiting and that elevation of these two substrates enables them to compete with FTase substrates for prenolation and thus allows sustained growth.
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Transformation of Candida albicans by electroporation.
There is disclosed a procedure for DNA-mediated transformation of Candida albicans by electroporation utilizing lithium acetate and dithiothreitol to weaken the cell wall structure and optimize the…
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Purification of geranylgeranyltransferase I from Candida albicans and cloning of the CaRAM2 and CaCDC43 genes encoding its subunits.
TLDR
The authors are investigating the role of geranylgeranylation in the related pathogenic yeast, Candida albicans, which is the most prevalent human fungal pathogen, and finding that hybrid GGTase I heterodimers are nonfunctional.
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Multiple origins of the green-sensitive opsin genes in fish
TLDR
In the LWS/MWS group, gene duplication of the ancestral red and green opsin genes predates the speciation between A. fasciatus and goldfish, suggesting that goldfish also has an additional gene which is orthologous to g101Af and g103Af.
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New fungal metabolite geranylgeranyltransferase inhibitors with antifungal activity
TLDR
From natural product screening, a number of azaphilones including one novel analog were isolated as broad-spectrum inhibitors of GGTase I and their inhibitory activities and antifungal activities are described.
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Citrafungins A and B, two new fungal metabolite inhibitors of GGTase I with antifungal activity.
TLDR
Citrafungins A and B are two new fungal metabolites of the alkylcitrate family that are inhibitors of GGTase I of various pathogenic fungal species with IC(50) values of 2.5-15 microM.
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Heterologous transformation of Zalerion arboricola
TLDR
This is the first report describing transformation of a member of the genus Zalerion, and using the niaD gene as a selectable marker provides a system for both positive and negative selection.
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