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The interaction of tubulin and other proteins with structure-stabilizing solvents☆
Abstract A study has been carried out of the interactions with proteins of solvent systems which are commonly used to stabilize proteins in solution or to crystallize proteins out of solution. The… Expand
Interaction of sodium dodecyl sulfate with tyrosyl chromophores in ribonuclease A and model compounds.
Abstract Ultraviolet difference spectra, solvent perturbation difference spectra, and temperature perturbation difference spectra indicate that tyrosyl residues of model compounds are affected by… Expand
Studies on bovine pancreatic ribonuclease A and model compounds in aqueous 2-methyl-2,4-pentanediol. I. Amino acid solubility, thermal reversibility of ribonuclease A, and preferential hydration of…
Abstract For X-ray investigations, RNase-A is crystallized from a mixture of 55% 2-methyl-2,4-pentanediol (MPD) and 45% water. Interactions of RNase-A and amino acids with this solvent have been… Expand
Abstract The effects of temperature, chelating agents, protein denaturants, and alpha- and beta-amylolysis on concanavalin A—polysaccharide complexes have been studied. Temperatures between 30 and… Expand
Interaction of ribonuclease A with aqueous 2-methyl-2,4-pentanediol at pH 5.8.
The interactions between ribonuclease A and solvent components in aqueous 2-methyl-2,4-pentanediol (MPD) have been investigated by differential refractometry and light scattering at pH 5.8, i.e.,… Expand
Effects of aqueous alcohol and other non-electrolyte solutions on the thermal perturbation spectra of aromatic compounds
Difference spectrophotometry of identical solutions of aromatic compounds at different temperatures results in thermal perturbation difference spectra. These difference spectra were examined over the… Expand
Interaction of sulfated polysaccharides with plasma membranes--II. Effect of ionic strength, temperature and trypsin digestion of the erythrocyte surface on the aggregation of erythrocytes by iota…
 Light scattering and differential refractometry
Publisher Summary This chapter presents the basic principles to discuss the problems and to describe the practical techniques involved in performing light scattering and differential refractometry… Expand
Alterations in hepatic microsomal proteins of mice administered mirex orally
Studies on bovine pancreatic ribonuclease A and model tyrosyl compounds in aqueous 2-methyl-2, 4-pentanediol. II. Spectral investigation of solvation.
Abstract Several spectroscopic techniques were used to study the states of solvation of the tyrosyls of bovine pancreatic ribonuclease A and tyrosyl model compounds in aqueous… Expand