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An alternative route to cyclic terpenes by reductive cyclization in iridoid biosynthesis
The discovery of iridoid synthase is reported, a plant-derived enzyme that generates the iridoids ring scaffold, as evidenced by biochemical assays, gene silencing, co-expression analysis and localization studies, and the prospects of using unrelated reductases to generate artificial cyclic scaffolds are highlighted. Expand
Development of Transcriptomic Resources for Interrogating the Biosynthesis of Monoterpene Indole Alkaloids in Medicinal Plant Species
Large-scale transcriptome sequence and expression profiles for three species of Asterids that produce medicinally important MIAs are generated, providing a rich resource for understanding plant specialized metabolism, and promotes realization of innovative production systems for plant-derived pharmaceuticals. Expand
Expression profiling of genes involved in paclitaxel biosynthesis for targeted metabolic engineering.
The preference for one side of the biosynthetic pathway branch in early taxane synthesis is revealed, where transcripts coding for TalphaH are abundant after elicitation with MJ but transcripts encoding the two enzymes for the alternative branch (TDAT and T10betaH) are not highly expressed following elicitation. Expand
Reengineering a tryptophan halogenase to preferentially chlorinate a direct alkaloid precursor.
It is demonstrated that the flavin-dependent halogenase RebH can be engineered to install chlorine preferentially onto tryptamine rather than the native substrate tryptophan, demonstrating the selectivity and potential utility of this mutant in metabolic engineering applications. Expand
Development of a Particle Bombardment‐Mediated Transient Transformation System for Taxus spp. Cells in Culture
This particle bombardment‐mediated transient transformation system was shown to be capable of transiently expressing the DsRed and β‐glucuronidase reporter genes under the control of the maize ubiquitin and CaMV 35S promoters, respectively and characterization of genes related to paclitaxel accumulation in culture can now proceed. Expand
High-Pressure Synthesis, Crystal Structure Determination, and a Ca Substitution Study of the Metallic Rhodium Oxide NaRh2O4
The sodium rhodate NaRh2O4 was synthesized for the first time and characterized by neutron and X-ray diffraction studies and measurements of magnetic susceptibility, specific heat, electricalExpand
TcJAMYC: a bHLH transcription factor that activates paclitaxel biosynthetic pathway genes in yew.
It is shown that the promoters of five genes encoding enzymes of the paclitaxel biosynthetic pathway are activated by MJ elicitation, suggesting that TcJAMYC could be engineered into Taxus cells to avoid the negative regulation of MJ-induced genes that follows initialMJ-induced positive regulation. Expand
WITHDRAWN: TcJAMYC: A bHLH transcription factor that activates paclitaxel biosynthetic pathway genes in yew.
This article was withdrawn by the authors before final publication on October 1, 2009 because the author did not believe the paper was fit for publication. Expand
Abstract C212: Discovery of high affinity cyclic peptidomimetics binding Mcl-1 and Ras.
Many well-validated oncology targets regulate signaling pathways through interactions with other proteins. Addressing these targets, which often lack suitable small molecule binding sites, requiresExpand