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Cell division: Mitotic kinases as regulators of cell division and its checkpoints
  • E. Nigg
  • Biology, Medicine
  • Nature Reviews Molecular Cell Biology
  • 2001
TLDR
An overview of the many mitotic kinases that regulate cell division and the fidelity of chromosome transmission is given. Expand
Plk4-induced centriole biogenesis in human cells.
TLDR
It is shown that overexpression of Polo-like kinase 4 (Plk4) in human cells induces centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole, and that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. Expand
Proteomic characterization of the human centrosome by protein correlation profiling
TLDR
A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins. Expand
Polo-like kinases and the orchestration of cell division
TLDR
Polo-like kinases are increasingly recognized as key regulators of mitosis, meiosis and cytokinesis and their targeting to different cellular structures through interactions with phosphorylated docking proteins is uncovered. Expand
Cep164, a novel centriole appendage protein required for primary cilium formation
TLDR
One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail and identified as an excellent marker for these structures. Expand
The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1
TLDR
It is reported that human Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2, indicating that Mst 2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway. Expand
Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis
TLDR
High-resolution mass spectrometry–based proteomics was applied to investigate the proteome and phosphoproteome of the human cell cycle on a global scale and quantified 6027 proteins and 20,443 unique phosphorylation sites and their dynamics, finding that nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylated site occupancy in mitosis, suggesting that these proteins may be inactivated by phosphorylate in mitotic cells. Expand
Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2
TLDR
The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment and it was shown that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Expand
The Polo kinase Plk4 functions in centriole duplication
TLDR
It is identified that Plk4 is required — in cooperation with Cdk2, CP110 and Hs-SAS6 — for the precise reproduction of centrosomes during the cell cycle and this findings provide an attractive explanation for the crucial function of PlK4 in cell proliferation. Expand
Centrosome aberrations: cause or consequence of cancer progression?
  • E. Nigg
  • Biology, Medicine
  • Nature Reviews Cancer
  • 1 November 2002
Many human tumours show centrosome aberrations, indicating an underlying deregulation of centrosome structure, duplication or segregation. Centrosomes organize microtubule arrays throughout the cellExpand
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