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Gene transfer into mouse lyoma cells by electroporation in high electric fields.
A simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed, according to which the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport. Expand
Electroporation and Electrofusion in Cell Biology
Cells in Electric Fields: Physical and Practical Electronic Aspects of Electro Cell Fusion and Electroporation and External Electric Field-Induced Transmembrane Potentials in Biological Systems: Features, Effects, and Optical Monitoring. Expand
Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.
Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses), and pulse duration plays a key role in the magnitude of the transfer. Expand
Fundamentals of electroporative delivery of drugs and genes.
The physico-chemical theory of ME and electroporative transport in terms of time-dependent flow coefficients has been developed to such a degree that analytical expressions are available to handle curvature and ionic strength effects on ME and transport. Expand
Permeability changes induced by electric impulses in vesicular membranes
Electric impulses were found to cause transient permeability changes in the membranes of vesicles storing biogenic amines, which may possibly represent a part of the sequence of events leading to stimulated neurohumoral secretion. Expand
Kinetics, statistics, and energetics of lipid membrane electroporation studied by molecular dynamics simulations.
A statistical theory is developed to facilitate direct comparison of experimental prepore formation kinetics with the (single event) preporation times derived from the simulations, which also allows us to extract an effective number of lipids involved in each pore formation event. Expand
Effective charge on acetylcholinesterase active sites determined from the ionic strength dependence of association rate constants with cationic ligands.
The high value of k120 and the space requirements of six to nine charged groups suggest that regions of the enzyme surface area larger than the catalytic sites themselves are effective in trapping cationic ligands. Expand
Electric field mediated gene transfer.
  • T. Wong, E. Neumann
  • Biology, Medicine
  • Biochemical and biophysical research…
  • 30 July 1982
Electric impulses in the intensity range of 5–10 kV/cm with a duration of 5-10 μs were found to appreciably increase the uptake of DNA into cells, which appears unique in its ease and simplicity. Expand
Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin alpha-bungarotoxin with Torpedo californica acetylcholine receptor.
The interaction dynamics of tetramethylrhodamin labelled alpha-bungarotoxin with the detergent solubilized nicotinic acetylcholine receptor (AChR) of Torpedo californica electric organ is characterized to determine low concentrations of slowly interacting molecules with different translational diffusion coefficients on the level of single molecule counting. Expand
Mechanism of electroporative dye uptake by mouse B cells.
The dye transport is described by Fick's first law, where, as a novelty, time-integrated flow coefficients are introduced, and the results of SERVA blue G cell coloring and the new analytical framework may also serve as a guideline for the optimization of the electroporative delivery of drugs that are similar in structure to SERVAblue G, for instance, bleomycin, which has been used successfully in the new discipline of electrochemotherapy. Expand