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Electroporation as a new technique for producing transgenic fish.
A recombinant plasmid, pMV-GH, containing rainbow trout growth hormone cDNA fused to mouse metallothionein I promoter, was introduced into medaka (Oryzias latipes) by electroporation. Of 3109Expand
Tauropine dehydrogenase from the marine sponge Halichondria japonica is a homolog of ornithine cyclodeaminase/mu-crystallin.
The partial amino acid sequence including the N- and C-terminal portions of tauropine dehydrogenase (EC from the marine sponge Halichondria japonica was determined by enzymatic cleavagesExpand
Occurrence of beta-alanine-specific opine dehydrogenase in the muscle of the limpet Cellana grata Gould (Archaeogastropoda).
The muscular tissues of the limpet Cellana grata exhibited beta-alanopine dehydrogenase (beta-AlDH) activity in addition to tauropine dehydrogenase (TaDH) activity and weak lactate dehydrogenaseExpand
Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea.
Alanopine dehydrogenase (AlDH) and three isoforms of strombine/alanopine dehydrogenase (St/AlDH) were purified from muscle tissue of the polychaete rockworm Marphysa sanguinea. The four enzymes,Expand
Presence of Free D-Amino Acids in Microalgae
Several species of microalgae (phytoplankton), 4 species of freshwater algae and 4 species of marine diatoms, were cultured germ-free in the laboratory. The presence of free D-amino acids wasExpand
Occurrence of free D-alanine in marine macroalgae
Occurrence of free D-alanine was verified in various marine macroalgae by reversed-phase HPLC analysis. The presence of free D-alanine was detected in 15 species (63%) out of the 24 species of algaeExpand
Purification and some properties of alanine racemase from the marine gastropod Cellana grata
High levels of free D-alanine were found in the muscle of a marine gastropod Cellana grata that inhabited the intertidal zone, and alanine racemase activity was detected in the muscle. The authorsExpand
Alanine racemase activity in the microalga thalassiosira sp.
In this paper, the authors report the detection of alanine racemase activity in the marine diatom Thalassiosira sp. Since the Thalassiosira sp. was cultured under germ-free conditions, it appearedExpand
A simple and rapid method for the analysis of fish histamine by paper electrophoresis
The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper,Expand