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Complete genome sequence of Neisseria meningitidis serogroup B strain MC58.
Neisseria meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system. Expand
Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing.
The entire genome sequence of a virulent serogroup B strain was used to identify vaccine candidates and allowed the identification of proteins that are surface exposed, that are conserved in sequence across a range of strains, and that induce a bactericidal antibody response, a property known to correlate with vaccine efficacy in humans. Expand
PCR for capsular typing of Haemophilus influenzae
PCR capsular typing overcomes the problems of cross-reaction and autoagglutination associated with the serotyping of H. influenzae strains. Expand
Capsular serotype-specific attack rates and duration of carriage of Streptococcus pneumoniae in a population of children.
The capsular serotype is a major determinant of both pneumococcal duration of carriage and attack rate and Published invasive odds ratios are a reliable and practical method of determining capsularserotype invasiveness and will be valuable for investigating and characterizing emerging Capsular serotypes in the context of conjugate vaccination. Expand
Adaptive evolution of highly mutable loci in pathogenic bacteria
It is argued that the coexistence within bacterial genomes of such ‘contingency’ genes with high mutation rates, and ‘housekeeping' genes with low mutation rates is the result of adaptive evolution, and facilitates the efficient exploration of phenotypic solutions to unpredictable aspects of the host environment while minimizing deleterious effects on fitness. Expand
Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species
Genetic exchange with related species sharing the same ecological niche is the main mechanism of evolution of S. pneumoniae, and the open pan-genome guarantees the species a quick and economical response to diverse environments. Expand
The molecular mechanism of phase variation of H. influenzae lipopolysaccharide
Multiple carbohydrate structures on the outer-membrane lipopolysaccharide (LPS) of the gram-negative pathogen H. influenzae undergo high frequency, reversible loss, indicative of phase variation.Expand
Meningococcal pilin: a glycoprotein substituted with digalactosyl 2,4‐diacetamido‐2,4,6‐trideoxyhexose
These studies have confirmed the previous observations that meningococcal pili are glycosylated and provided the first structural evidence for the presence of covalently linked carbohydrate on pili, and revealed a completely novel protein/saccharide linkage. Expand
Sialic acid in the lipopolysaccharide of Haemophilus influenzae: strain distribution, influence on serum resistance and structural characterization
It is demonstrated that sialic acid influences resistance to the killing effect of normal human serum but has little effect on attachment to, or invasion of, cultured human epithelial cells or neutrophils. Expand
Phosphorylation of the Lipid A Region of Meningococcal Lipopolysaccharide: Identification of a Family of Transferases That Add Phosphoethanolamine to Lipopolysaccharide
ABSTRACT A gene, NMB1638, with homology to the recently characterized gene encoding a phosphoethanolamine transferase, lpt-3, has been identified from the Neisseria meningitidis genome sequence andExpand