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Brucella Evades Macrophage Killing via VirB-dependent Sustained Interactions with the Endoplasmic Reticulum
TLDR
It is shown in a model of Brucella abortus infection of murine bone marrow–derived macrophages that a fraction of the bacteria that survive an initial macrophage killing proceed to replicate in a compartment segregated from the endocytic pathway. Expand
Brucella abortus Transits through the Autophagic Pathway and Replicates in the Endoplasmic Reticulum of Nonprofessional Phagocytes
TLDR
The results are compatible with the hypothesis that pathogenic B. abortus exploits the autophagic machinery of HeLa cells to establish an intracellular niche favorable for its replication within the ER. Expand
Brucella intracellular life: from invasion to intracellular replication.
TLDR
The integrity of BrucellA LPS on the bacterial surface is one of the required factors for Brucella intracellular survival, and therefore for virulence. Expand
MyD88, but Not Toll-Like Receptors 4 and 2, Is Required for Efficient Clearance of Brucella abortus
TLDR
It is found that during infection of macrophages, Brucella avoids activation of Toll-like receptor 4 at 6 h but activates TLR4, TLR2, and myeloid differentiation factor 88 (MyD88) at 24 h postinfection, and even though its activation is delayed, MyD88 is important for host defense against BrucellA infection in vivo. Expand
Rough vaccines in animal brucellosis: structural and genetic basis and present status.
TLDR
Rough mutants obtained by molecular biology methods on the knowledge of the genetics and structure of Brucella lipopolysaccharide may offer alternatives, and analyses in mice suggest that mutations affecting only the O-polysaccharides result in better vaccines than those affecting both core and O- polysaccharid. Expand
Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria
TLDR
The chemical analysis of the lipid A fraction revealed that Brucella species contain both glucosamine and diaminoglucose, thus suggesting the presence of a so-called mixed lipid A type, and the serological analysis with polyclonal and monoclonal antibodies is in agreement with the existence of mixed lipid B type in B. abortus. Expand
Brucella evolution and taxonomy.
TLDR
A critical reappraisal of the species concept, a review of the population structure of bacteria and the analysis of Brucella genetic diversity by methods other than DNA-DNA hybridization show that there are no scientific grounds to apply the genomospecies concept to this genus. Expand
Virulent Brucella abortus Prevents Lysosome Fusion and Is Distributed within Autophagosome-Like Compartments
TLDR
Virulent and attenuated Brucella abortus strains attach to and penetrate nonprofessional phagocytic HeLa cells and prevent lysosome-phagosome fusion and were found distributed in the perinuclear region within compartments resembling autophagosomes. Expand
Brucella abortus Uses a Stealthy Strategy to Avoid Activation of the Innate Immune System during the Onset of Infection
TLDR
It is proposed that Brucella has developed a stealth strategy through PAMPs reduction, modification and hiding, ensuring by this manner low stimulatory activity and toxicity for cells. Expand
Brucella ceti and Brucellosis in Cetaceans
TLDR
Since the first case of brucellosis detected in a dolphin aborted fetus, an increasing number of Brucella ceti isolates has been reported in members of the two suborders of cetaceans: Mysticeti and Odontoceti, and three different groups have been recognized according to their preferred host, bacteriological properties, and distinct genetic traits. Expand
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