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Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed…
Findings confirm that the TmDGAT1 gene encodes an acyl-CoA-dependent DGAT1, and alteration of this putative serine/threonine protein kinase site can be exploited to enhance DGat1 activity, and expression of mutated DGAT2 can be used to enhance oil content.
Unusual sequence relationships between two isolates of citrus tristeza virus.
- M. Mawassi, E. Mietkiewska, R. Gofman, G. Yang, M. Bar-joseph
- BiologyThe Journal of general virology
- 1 September 1996
The observed deviation between the large RNA genomes of the two CTV strains is greater than that among different viruses of most other groups, raising the question of how to define the taxonomy of these viruses.
Seed-expressed fluorescent proteins as versatile tools for easy (co)transformation and high-throughput functional genomics in Arabidopsis.
- A. Stuitje, E. C. Verbree, K. H. van der Linden, E. Mietkiewska, J. Nap, T. Kneppers
- BiologyPlant biotechnology journal
- 1 July 2003
It is demonstrated that fluorescent proteins can be used as visual selection markers for the transformation of Arabidopsis thaliana by the floral dip method, and this technology, of tagging and identifying transformed seeds by fluorescence provides a novel high-throughput screening system with many potential applications in plant biotechnology.
Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution.
- V. Katavić, E. Mietkiewska, D. Barton, E. M. Giblin, D. Reed, D. Taylor
- Biology, MedicineEuropean journal of biochemistry
- 1 November 2002
For the first time, the low erucic acid trait in canola B. napus can be attributed to a single amino acid substitution which prevents the biosynthesis of the eicosenoic and Erucic acids.
Defective RNA molecules associated with citrus tristeza virus.
Northern hybridization analysis demonstrated that this 2.4-kb defective RNA is an abundant species among the other CTV-specific ss- and ds-RNAs in infected plants.
Brassica carinata – a new molecular farming platform for delivering bio‐industrial oil feedstocks: case studies of genetic modifications to improve very long‐chain fatty acid and oil content in seeds
This collaborative B. carinata breeding/germplasm development/value‐added molecular modification effort will not only contribute to the development of renewable feedstocks for the emerging Canadian bioeconomy (biorefinery/bioproducts), but also promises to generate positive economic and environmental benefits.
Multiple species of defective RNAs in plants infected with citrus tristeza virus.
Northern blot hybridizations of dsRNAs extracted from serial passages of the Israeli VT isolate (CTV-VT) and from different plants infected with a single source of inoculum showed considerable variation both in the presence and in the relative abundance of the defective RNA (D-RNA) bands.
Identification of a Pair of Phospholipid:Diacylglycerol Acyltransferases from Developing Flax (Linum usitatissimum L.) Seed Catalyzing the Selective Production of Trilinolenin*
- Xue Pan, Rodrigo M. P. Siloto, Aruna D. Wickramarathna, E. Mietkiewska, R. Weselake
- BiologyThe Journal of Biological Chemistry
- 2 July 2013
This study indicated the existence of unique PDAT enzymes from flax that are able to preferentially catalyze the synthesis of TAG containing ALA acyl moieties.
Seed-Specific Heterologous Expression of a Nasturtium FAE Gene in Arabidopsis Results in a Dramatic Increase in the Proportion of Erucic Acid1
Results indicate that the nasturtium FAE gene encodes a condensing enzyme involved in the biosynthesis of very long chain fatty acids, utilizing monounsaturated and saturated acyl substrates.
Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1Δ mutant strain of Saccharomyces cerevisiae
- E. Mietkiewska, Rodrigo M. P. Siloto, J. Dewald, Saleh Shah, D. Brindley, R. Weselake
- BiologyThe FEBS journal
- 1 March 2011
Evidence is provided that Arabidopsis and B.’napus PAHs restore lipid synthesis in yeast and that DXDXT is a functional enzymic motif within plant PAH's.