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Bioluminescent yeast assays for detecting estrogenic and androgenic activity in different matrices.
In this paper we describe the construction and use of a set of bioluminescent yeast strains for the detection of compounds that can affect androgen or estrogen receptor mediated hormonal signalling.Expand
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A new recombinant cell-based bioluminescent assay for sensitive androgen-like compound detection.
A public concern is continuously arising about the presence of natural and anthropogenic compounds which affect human health by modulating normal endocrine functions. These substances, defined asExpand
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Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications.
Light emission from the North American firefly Photinus pyralis, which emits yellow-green (557-nm) light, is widely believed to be the most efficient bioluminescence system known, making thisExpand
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A 3D-printed device for a smartphone-based chemiluminescence biosensor for lactate in oral fluid and sweat.
Increasingly, smartphones are used as portable personal computers, revolutionizing communication styles and entire lifestyles. Using 3D-printing technology we have made a disposable minicartridgeExpand
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Field-flow fractionation and biotechnology.
The gentle separation mechanism has made field-flow fractionation particularly suited to samples of biotechnological interest, from proteins and nucleic acids to viruses, subcellular units and wholeExpand
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Bioluminescence in analytical chemistry and in vivo imaging
Progress in molecular biology has made available new bioanalytical tools that take advantage of the great detectability and the simple analytical format of bioluminescence. Combining luminescentExpand
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Analytical strategies for improving the robustness and reproducibility of bioluminescent microbial bioreporters
AbstractWhole-cell bioluminescent (BL) bioreporter technology is a useful analytical tool for developing biosensors for environmental toxicology and preclinical studies. However, when applied to realExpand
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One-step triplex-polymerase chain reaction assay for the authentication of yellowfin (Thunnus albacares), bigeye (Thunnus obesus), and skipjack (Katsuwonus pelamis) tuna DNA from fresh, frozen, and
A one-step triplex-polymerase chain reaction (PCR)-based assay was developed to discriminate between three tuna species, Thunnus albacares, Thunnus obesus, and Katsuwonus pelamis, even in highlyExpand
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Biotechnological applications of bioluminescence and chemiluminescence.
Recent progress in molecular biology has made available several biotechnological tools that take advantage of the high detectability and rapidity of bioluminescence and chemiluminescenceExpand
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A portable bioluminescence engineered cell-based biosensor for on-site applications.
We have developed a portable biosensing device based on genetically engineered bioluminescent (BL) cells. Cells were immobilized on a 4 × 3 multiwell cartridge using a new biocompatible matrix thatExpand
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