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Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory
TLDR
Differences in the cell type-specific expression of mediators of SARS-CoV-2 viral entry may be responsible for aspects of COVID-19 epidemiology and clinical course, and point to putative molecular pathways involved in disease susceptibility and pathogenesis.
Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity
TLDR
It is shown that the spike protein of Omicron has a higher affinity for ACE2 compared with Delta, and a marked change in its antigenicity increases OmicRON’s evasion of therapeutic monoclonal and vaccine-elicited polyclonal neutralizing antibodies after two doses.
scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation
The Human Cell Atlas is a large international collaborative effort to map all cell types of the human body. Single-cell RNA sequencing can generate high-quality data for the delivery of such an
Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics
TLDR
An integrated analysis of over 100 single-cell and single-nucleus transcriptomics studies illustrates severe acute respiratory syndrome coronavirus 2 viral entry gene coexpression patterns across different human tissues, and shows association of age, smoking status and sex with viralEntry gene expression in respiratory cell populations.
Characterization and target genes of nine human PRD-like homeobox domain genes expressed exclusively in early embryos
TLDR
The data implicate a role for these previously uncharacterized PRD-like homeodomain proteins in the regulation of human embryo genome activation and preimplantation embryo development.
The human PRD-like homeobox gene LEUTX has a central role in embryo genome activation
TLDR
Using a human embryonic stem cell overexpression model, it is shown that the complete homeodomain isoform is functional and sufficient to activate the transcription of a large proportion of the genes that are upregulated in human embryo genome activation (EGA).
Local and systemic responses to SARS-CoV-2 infection in children and adults
TLDR
Analysis of paediatric and adult patients with COVID-19 as well as healthy control individuals using single-cell multi-omic profiling of matched nasal, tracheal, bronchial and blood samples provides evidence that dendritic cells initiate interferon signalling in early infection, and identifies epithelial cell states associated with CO VID-19 and age.
Novel PRD-like homeodomain transcription factors and retrotransposon elements in early human development
TLDR
High-resolution RNA sequencing of 348 oocytes, zygotes and single blastomeres from 2- to 3-day-old embryos provides a detailed analysis of the human preimplantation transcriptome and identifies significantly enriched gene regulatory motifs that often overlap with Alu elements.
SARS-CoV-2 Omicron spike mediated immune escape and tropism shift
TLDR
Omicron appears to have gained significant evasion from neutralising antibodies whilst maintaining sensitivity to antiviral drugs targeting the polymerase, with implications for altered pathogenesis.
Molecular phenotyping reveals the identity of Barrett’s esophagus and its malignant transition
TLDR
A single-cell approach to investigate the cell of origin for Barrett's esophagus (BE) and the mechanisms leading to the development of esophageal adenocarcinoma (EAC) revealed that BE originates from the gastric cardia and that EAC arises from undifferentiated BE cells.
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