• Publications
  • Influence
Biocatalytic approaches applied to the synthesis of nucleoside prodrugs.
The aim of this review is to give an overview of the opportunities provided by biocatalytic procedures in the preparation of nucleoside prodrugs, and the potential ofBiocatalysis in this research area will be presented. Expand
Developing a Collection of Immobilized Nucleoside Phosphorylases for the Preparation of Nucleoside Analogues: Enzymatic Synthesis of Arabinosyladenine and 2,3-Dideoxyinosine
The use of nucleoside phosphorylases (NPs; EC 2.4.2.n) represents a convenient alternative to the chemical route for the synthesis of natural and modified nucleosides. We purified four recombinantlyExpand
Screening of Catalytically Active Microorganisms for the Synthesis of 6-Modified Purine Nucleosides
An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy, 6-iodo- and 6-mercaptopurine ribonucleoside. Expand
New and highly active microbial phosphotriesterase sources.
In this study, a simple and sensitive fluorimetric screening method on solid media was employed that allowed the selection of six strains with phosphotriesterase activity to be selected, afforded notable results, with the most remarkable one the increased activity exhibited by Nocardia asteroides and Streptomyces setonii strains at 50°C, 7 and 30 times higher than at 30°C. Expand
Microbial degradation of organophosphorus pesticides using whole cells and enzyme extracts
Results suggest that according to the circumstances, immobilized whole cells (avoiding cellular disruption and centrifugation) or enzyme extracts (which can be handled more easily) could be used. Expand
Synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts.
Enterobacter gergoviae and Arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (AraG), an efficient anti leukemic drug. Expand
Immobilized Escherichia coli BL21 as a Catalyst for the Synthesis of Adenine and Hypoxanthine Nucleosides
The transglycosylation reaction involved in the synthesis of adenosine from uridine and adenine was chosen as a model system to study the characteristics of these biocatalysts and whole cells immobilized on agarose proved to be optimal and could be used up to 30 times without significant loss of activity. Expand
Microbial synthesis of 2,6-diaminopurine nucleosides
Abstract 2,6-Diaminopurine nucleosides are used as pharmaceutical drugs or prodrugs against cancer and viral diseases. The synthesis of 2,6-diaminopurine riboside, -2′-deoxyriboside,Expand
Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
This is the first report of a biocatalyst exhibiting opposite redox transformations of the carboxylic acid moiety in benzoic acid derivatives, namely oxidative decarboxylation and carboxyl reduction, in a structure-dependent fashion. Expand
Microbial Synthesis of Antiviral Nucleosides Using Escherichia coli BL21 as Biocatalyst
Experimental conditions such as shaking (aeration) rate, concentration of reagents and extent of culture growth for the optimal synthesis of adenosine using Escherichia coli BL21 as biocatalyst wereExpand