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Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis.
The spacer oligonucleotide typing (spoligotyping) method was evaluated for its ability to differentiate Mycobacterium bovis strains. This method detects the presence or absence of spacers of theExpand
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Characterization of Pathogenic Vibrio parahaemolyticus Isolates from Clinical Sources in Spain and Comparison with Asian and North American Pandemic Isolates
ABSTRACT In spite of the potential risk involved with contamination of seafood with Vibrio parahaemolyticus, there is a lack of information on the occurrence of pathogenic V. parahaemolyticus inExpand
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Detection of Salmonella spp., Yersinia enterocolitica and verocytotoxin-producing Escherichia coli O157 in pigs at slaughter in Italy.
From December 1999 to December 2000, 150 pigs were randomly selected in two large abattoirs of northern Italy. Caecal material and carcass swabs were collected and examined for Salmonella, YersiniaExpand
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Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain.
Isolates from the Mycobacterium tuberculosis complex cultured from caprine pathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrateExpand
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Replicon Typing of Plasmids Carrying CTX-M or CMY β-Lactamases Circulating among Salmonella and Escherichia coli Isolates
ABSTRACT Replicon typing of plasmids carrying blaCTX-M or blaCMY β-lactamase genes indicates a predominance of I1 and A/C replicons among blaCMY-carrying plasmids and five different plasmid scaffoldsExpand
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Simple and rapid detection of Mycobacterium tuberculosis complex organisms in bovine tissue samples by PCR.
Mycobacterium bovis is a slowly growing microorganism, and confirmation of the diagnosis by conventional culture is a lengthy process. A simple, rapid method for the extraction of DNA from bovineExpand
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Longitudinal Farm Study of Extended-Spectrum β-Lactamase-Mediated Resistance
ABSTRACT Extended-spectrum β-lactamase (ESBL)-mediated resistance is of considerable importance in human medicine. Recently, such enzymes have been reported in bacteria from animals. We describe aExpand
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Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting
ABSTRACT Reliable and sufficiently discriminative methods are needed for differentiating individual strains of Salmonella entericaserotype Enteritidis beyond the phenotypic level; however, aExpand
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Pathology of naturally occurring bovine tuberculosis in England and Wales.
The aim of this study was to obtain a contemporary data set of pathology in tuberculin reactor and in-contact cattle in England and Wales. Four hundred animals (200 reactors and 200 in-contacts) fromExpand
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Evaluation of Four DNA Typing Techniques in Epidemiological Investigations of Bovine Tuberculosis
ABSTRACT DNA fingerprinting techniques were used to type 273 isolates ofMycobacterium bovis from Australia, Canada, the Republic of Ireland, and Iran. The results of restriction fragment lengthExpand
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