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The intracisternal A-particle gene family: structure and functional aspects.
Publisher Summary This chapter reviews the various aspects of research on intracisternal type A particles (IAPs)—expression in early development, genomic sequences as chromosomal genes andExpand
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EBP-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single- to double-strand transitions in DNA.
We have previously reported the purification and characterization of the transcription factor EBP-80 (Falzon, M., and Kuff, E. L. (1989) J. Biol. Chem. 264, 21915-21922). EBP-80 mediates the DNAExpand
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Nucleotide sequence of a complete mouse intracisternal A-particle genome: relationship to known aspects of particle assembly and function.
The 7,095-nucleotide sequence of a mouse genomic intracisternal A-particle (IAP) element, MIA14, is reported. MIA14 is known to be colinear with IAP 35S RNA and to contain functional long terminalExpand
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ISOLATION OF NUCLEI FROM MAMMALIAN TISSUES THROUGH THE USE OF TRITON X-100
  • W. Hymer, E. Kuff
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry…
  • 1 May 1964
It has been found that nuclei from a variety of mammlian cell types maintain their structural integrity (at the light microscope level) when exposed to the non-ionic detergent Triton X-100, in theExpand
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DNA-dependent protein kinase is activated by nicks and larger single-stranded gaps.
DNA-PK is a DNA-activated serine/threonine protein kinase capable of phosphorylating a number of nuclear DNA-binding proteins. Purified human DNA-PK has two subunits, a 350-kDa polypeptide, Prkdc,Expand
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Binding of the transcription factor EBP-80 mediates the methylation response of an intracisternal A-particle long terminal repeat promoter.
Intracisternal A-particle (IAP) expression in mouse cells has been correlated with hypomethylation of HhaI and HpaII sites in proviral long terminal repeats (LTRs). In a previous study, in vitroExpand
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Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA willExpand
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Intracisternal A-particle genes as movable elements in the mouse genome.
We analyzed two functionally defective mouse kappa light chain gene variants previously shown to contain novel insertions of repetitive DNA in their intervening sequences [Hawley, R. G., Shulman, M.Expand
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Selective activation of a discrete family of endogenous proviral elements in normal BALB/c lymphocytes.
Intracisternal A-particle (IAP) proviral elements are abundant and widely dispersed in the mouse genome. IAP-related transcripts have been detected in normal mouse tissues where expression is underExpand
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The long terminal repeat of an endogenous intracisternal A-particle gene functions as a promoter when introduced into eucaryotic cells by transfection.
We describe experiments designed to determine whether an endogenous intracisternal A-particle (IAP) gene randomly selected from a mouse embryo library has the potential to be transcriptionallyExpand
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