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- Publications
- Influence
P. aeruginosa SGNH Hydrolase-Like Proteins AlgJ and AlgX Have Similar Topology but Separate and Distinct Roles in Alginate Acetylation
The O-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. Pseudomonas aeruginosa secretes the anionic, O-acetylated… Expand
Functional properties of the carboxy-terminal host cell-binding domains of the two toxins, TcdA and TcdB, expressed by Clostridium difficile.
- T. Dingle, Stefanie Wee, +8 authors G. Armstrong
- Biology, Medicine
- Glycobiology
- 1 September 2008
The biological and ligand-binding properties of recombinant C-terminal cell-binding domains (CBDs) and subdomains of the two large exotoxins, Toxin A (TcdA) and Toxin B (TcdB) expressed by… Expand
Influence of coulombic repulsion on the dissociation pathways and energetics of multiprotein complexes in the gas phase
- Igor Sinelnikov, E. Kitova, J. Klassen
- Chemistry, Medicine
- Journal of the American Society for Mass…
- 1 April 2007
Thermal dissociation experiments, implemented with blackbody infrared radiative dissociation and Fourier-transform ion cyclotron resonance mass spectrometry, are performed on gaseous protonated and… Expand
Gangliosides are Ligands for Human Noroviruses
- Ling Han, M. Tan, M. Xia, E. Kitova, X. Jiang, J. Klassen
- Chemistry, Medicine
- Journal of the American Chemical Society
- 8 August 2014
Human noroviruses (NoVs) are known to recognize histo-blood group antigens (HBGAs) as attachment factors. We report the first experimental evidence that sialic acid-containing glycosphingolipids… Expand
Thermal decomposition of a gaseous multiprotein complex studied by blackbody infrared radiative dissociation. Investigating the origin of the asymmetric dissociation behavior.
- N. Felitsyn, E. Kitova, J. Klassen
- Chemistry, Medicine
- Analytical chemistry
- 30 August 2001
The blackbody infrared radiative dissociation technique was used to study the thermal decomposition of the gaseous B5 pentamer of the Shiga-like toxin I and its complexes with the Pk trisaccharide… Expand
Method for distinguishing specific from nonspecific protein-ligand complexes in nanoelectrospray ionization mass spectrometry.
- Jiangxiao Sun, E. Kitova, W. Wang, J. Klassen
- Chemistry, Medicine
- Analytical chemistry
- 1 April 2006
A new methodology for distinguishing between specific and nonspecific protein-ligand complexes in nanoelectrospray ionization mass spectrometry (nanoES-MS) is described. The method involves the… Expand
Quantifying Protein-Fatty Acid Interactions Using Electrospray Ionization Mass Spectrometry
- L. Liu, E. Kitova, J. Klassen
- Chemistry, Medicine
- Journal of the American Society for Mass…
- 1 February 2011
The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH3(CH2)xCOOH,… Expand
Optogenetic control with a photocleavable protein, PhoCl
- Wei Zhang, A. Lohman, +10 authors R. E. Campbell
- Biology, Medicine
- Nature Methods
- 1 April 2017
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced… Expand
Stability of the homopentameric b subunits of shiga toxins 1 and 2 in solution and the gas phase as revealed by nanoelectrospray fourier transform ion cyclotron resonance mass spectrometry
- E. Kitova, Rambod Daneshfar, P. Marcato, G. Mulvey, G. Armstrong, J. Klassen
- Chemistry, Medicine
- Journal of the American Society for Mass…
- 1 December 2005
The assembly of the B subunits of Shiga toxins (Stx) 1 and 2 and the influence of solution conditions (protein concentration, temperature, pH, and ionic strength) on it are investigated using… Expand
Catalytic Mechanism and Mode of Action of the Periplasmic Alginate Epimerase AlgG*
- F. Wolfram, E. Kitova, +4 authors P. Howell
- Biology, Medicine
- The Journal of Biological Chemistry
- 7 January 2014
Background: The alginate epimerase AlgG converts mannuronate to its C5 epimer guluronate at the polymer level. Results: The structure of Pseudomonas syringae AlgG has been determined, and the protein… Expand