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P bodies: at the crossroads of post-transcriptional pathways
The available evidence indicates that P bodies are sites where mRNAs that are not being translated accumulate, the information carried by associated proteins and regulatory RNAs is integrated, and their fate — either translation, silencing or decay — is decided. Expand
P-Body Formation Is a Consequence, Not the Cause, of RNA-Mediated Gene Silencing
It is shown that processes such as mRNA decay, NMD, and RNA-mediated gene silencing are functional in cells lacking detectable microscopic P bodies, indicating that P bodies arise as a consequence of silencing. Expand
Gene silencing by microRNAs: contributions of translational repression and mRNA decay
This work has shown that microRNAs can induce mRNA degradation in animals and, conversely, translational repression in plants and shed light on the specific mechanisms of target silencing. Expand
mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes.
It is shown that depletion of GW182 leads to changes in mRNA expression profiles strikingly similar to those observed in cells depleted of the essential Drosophila miRNA effector AGO1, indicating that GW182 functions in the miRNA pathway. Expand
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus
The GTPase Ran is essential for nuclear import of proteins with a classical nuclear localization signal (NLS). Ran's nucleotide‐bound state is determined by the chromatin‐bound exchange factor RCC1Expand
A Novel Class of RanGTP Binding Proteins
It is proposed that RanBP7 might represent a nuclear transport factor that carries an as yet unknown cargo, which could apply as well for this entire class of related RanGTP-binding proteins. Expand
Towards a molecular understanding of microRNA-mediated gene silencing
Understanding of the mechanisms of silencing is enhanced, making it possible to describe in molecular terms a continuum of direct interactions from miRNA target recognition to mRNA deadenylation, decapping and 5′-to-3′ degradation. Expand
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator and is the second cellular RNA binding protein shown to be directly involved in the export of its target RNA. Expand
The spliceosome deposits multiple proteins 20–24 nucleotides upstream of mRNA exon–exon junctions
It is reported that the spliceosome stably deposits several proteins on mRNAs, probably as a single complex of ∼335 kDa, which protects 8 nucleotides of mRNA from complete RNase digestion at a conserved position 20–24 nucleotide upstream of exon–exon junctions. Expand
The exon–exon junction complex provides a binding platform for factors involved in mRNA export and nonsense‐mediated mRNA decay
The composition of the exon–exon junction complex is dynamic in vivo and is subject to significant evolution upon mRNA export to the cytoplasm. Expand