• Publications
  • Influence
Dual targeting of a single tRNA(Trp) requires two different tryptophanyl-tRNA synthetases in Trypanosoma brucei.
TLDR
RNA interference analysis established that both Trp RS1 and TrpRS2 are essential for growth and required for cytosolic and mitochondrial tryptophanyl-tRNA formation, respectively, and the notion that, in an organism, all nuclear-encoded tRNAs assigned to a given amino acid are charged by a single aminoacyl-t RNA synthetase is not universally valid. Expand
In vitro import of proteins into mitochondria of Trypanosoma brucei and Leishmania tarentolae.
TLDR
Novel procedures for the isolation of mitochondria from two trypanosomatid species and trypsin pretreatment abolished the ability of the mitochondria to import proteins, indicating the involvement of proteinaceous components at the surface of mitochondrialria. Expand
Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
TLDR
It is shown that unexpectedly a fraction of elongator tRNAMet becomes formylated after import into mitochondria, and RNA interference assays identify the gene encoding the trypanosomal formylase activity. Expand
Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes
TLDR
The results show that, because of the extensive remodeling, the trypanosomal translational machinery adopted unusual solutions to accomplish some basic protein synthesis mechanisms, and this work determined the atomic structure of the mitoribosome from T. brucei using cryo–electron microscopy. Expand
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery
TLDR
The assembly of the small mitoribosomal subunit in Trypanosoma brucei involves a large number of factors and proceeds through the formation of assembly intermediates, which are analyzed by using cryo–electron microscopy. Expand
Non-canonical Eukaryotic Glutaminyl- and Glutamyl-tRNA Synthetases Form Mitochondrial Aminoacyl-tRNA in Trypanosoma brucei*
TLDR
This work identifies genes encoding glutaminyl- and glutamyl-tRNA synthetase in the closely related organism Trypanosoma brucei and indicates an adaptation to the situation in which the cytosol and mitochondria use the same set of tRNAs. Expand
tRNAs and proteins are imported into mitochondria of Trypanosoma brucei by two distinct mechanisms.
TLDR
Results show that tRNAs and proteins in T. brucei are imported by fundamentally different mechanisms. Expand
Dual Targeting of a tRNAAsp Requires Two Different Aspartyl-tRNA Synthetases in Trypanosoma brucei*
TLDR
Mitochondrial Tb-AspRS2 defines a novel group of eukaryotic AspRSs with an expanded substrate specificity that are restricted to trypanosomatids and therefore may be exploited as a novel drug target. Expand
Isolation of mitochondria from procyclic Trypanosoma brucei.
TLDR
The two most commonly used methods to isolate mitochondria from insect stage T. brucei are described and discussed. Expand
...
1
2
...