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Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesiss⃞s⃞ The online version of this article (available at http://www.jlr.org) contains an additional
TLDR
DHA synthesis in stearidonic acid-fed yeast is successfully implemented by coexpression of the Δ6- and Δ5-elongases from T. tauri and O. pseudonana with the Δ5 and Δ4-desaturases from two other algae.
Peroxisomal Acyl-CoA Synthetase Activity Is Essential for Seedling Development in Arabidopsis thaliana
TLDR
It is demonstrated that peroxisomal LACS activity and the PXA1 transporter are essential for early seedling growth.
The evolution of desaturases.
Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants
TLDR
The transgenic production of significant amounts of AA and EPA in Brassica juncea seeds via a stepwise metabolic engineering strategy is reported, demonstrating the practical feasibility of large-scale production of this important ω-3 fatty acid in oilseed crops.
Defensins from Insects and Plants Interact with Fungal Glucosylceramides*
TLDR
Structurally homologous anti-fungal peptides present in species from different eukaryotic kingdoms interact with the same target in the fungal plasma membrane, namely glucosylceramides, and as such support the hypothesis that defensins from plants and insects have evolved from a single precursor.
Biosynthesis of Very-Long-Chain Polyunsaturated Fatty Acids in Transgenic Oilseeds: Constraints on Their Accumulationw⃞
TLDR
The successful production of VLCPUFA in transgenic oilseeds and the identification of constraints on their accumulation are indicated, which indicate alternative strategies to circumvent this bottleneck.
Glucosylceramide Synthases, a Gene Family Responsible for the Biosynthesis of Glucosphingolipids in Animals, Plants, and Fungi*
TLDR
A systematic functional analysis of a glycosyltransferase gene family with members of animal, plant, fungal, and bacterial origin found five novel glucosylceramide synthase genes were identified from the plant Gossypium arboreum, the nematode Caenorhabditis elegans, and the fungi Magnaporthe grisea, Candida albicans, and P. pastoris.
Functional characterization of β-ketoacyl-CoA synthase genes from Brassica napus L.
TLDR
The Bn-FAE1.1 gene from Askari showed seed-specific expression in transgenic rapeseed plants and over-expression of the coding region of the Askari gene in developing seeds of transgenic Drakkar plants resulted in a significant increase in the levels of eicosenoic acid and erucic acid esterified in the seed oil.
Cholesterol glucosylation promotes immune evasion by Helicobacter pylori
TLDR
A mechanism regulating the host–pathogen interaction whereby glucosylation of a lipid tips the scales towards immune evasion or response is proposed, which corroborates the importance of cholesteryl glucosides for escaping phagocytosis, T cell activation and bacterial clearance in vivo.
Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis.
TLDR
This is the first report describing the isolation and biochemical characterization of fatty acid desaturases from diatoms and indicates that these two Desaturases functioned in the omega3- and omega6-pathways, in good agreement with both routes coexisting in Phaeodactylum tricornutum.
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