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Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.
A biosynthetic antibody binding site, which incorporated the variable domains of anti-digoxin monoclonal antibody 26-10 in a single polypeptide chain (Mr = 26,354), was produced in Escherichia coliExpand
The kinetics of formation of native ribonuclease during oxidation of the reduced polypeptide chain.
In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity appeared after the sample of bovine pancreatic ribonuclease wasExpand
Binding of immunogenic peptides to Ia histocompatibility molecules
Most cellular interactions essential for the development of an immune response involve the membrane glycoproteins encoded in the major histocompatibility gene complex1–3. The products of the IExpand
Application of a radioimmunoassay for angiotensin I to the physiologic measurements of plasma renin activity in normal human subjects.
ABSTRACT A radioimmunoassay for angiotensin I and its application to the determination of renin activity is described. The assay employs antibodies raised to copolymers of angiotensin I andExpand
Promotion of vascular smooth muscle cell growth by homocysteine: a link to atherosclerosis.
Plasma homocysteine levels are elevated in 20-30% of all patients with premature atherosclerosis. Although elevated homocysteine levels have been recognized as an independent risk factor forExpand
Cloning and Functional Analysis of the Promoter for KDR/flk-1, a Receptor for Vascular Endothelial Growth Factor(*)
KDR/flk-1 is one of two receptors for vascular endothelial growth factor, a potent angiogenic peptide. KDR/flk-1 is an early marker for endothelial cell progenitors, and its expression is restrictedExpand
Studies on the reduction and re-formation of protein disulfide bonds.
The information contained in the amino acid sequence of a protein is linear, such as it is in DNA, but proteins function by virtue of the arrangement of their linear structure into a well-definedExpand
Protein engineering of single-chain Fv analogs and fusion proteins.
Publisher Summary This chapter describes the minimal antibody binding site through the protein engineering of single-chain Fv analog and fusion proteins. In this approach, the genes encoding VH andExpand
Antigenic determinants in proteins coincide with surface regions accessible to large probes (antibody domains).
We evaluated surface areas on proteins that would be accessible to contacts with large (1-nm radius) spherical probes. Such spheres are comparable in size to antibody domains that containExpand
Induction of Apoptosis by Pyrrolidinedithiocarbamate and N-Acetylcysteine in Vascular Smooth Muscle Cells (*)
  • J. Tsai, M. Jain, +9 authors M. Lee
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • 16 February 1996
Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC andExpand