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The photon counting histogram in fluorescence fluctuation spectroscopy.
Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelationExpand
Lipid rafts reconstituted in model membranes.
The notion that in biomembranes selected lipids could laterally aggregate to form more ordered, detergent-resistant lipid rafts into which glycosphingolipid- and cholesterol-rich lipid domains partition is strongly supported by this study. Expand
Measuring fast dynamics in solutions and cells with a laser scanning microscope.
Novel extensions to ICS are developed that probe spatial correlations in previously inaccessible temporal windows and are used to determine for the first time spatially resolved diffusions of paxillin-EGFP stably expressed in CHOK1 cells. Expand
Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence.
It is concluded that the presence of water molecules in the phospholipid matrix are responsible for the spectral properties of Laurdan in the gel phase. Expand
Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope
A technique based on moment-analysis for the measurement of the average number of molecules and brightness in each pixel in fluorescence microscopy images is described, which reveals binding dynamics at the focal adhesions with pixel resolution and provides a picture of the binding and unbinding process. Expand
Investigation of human brain hemodynamics by simultaneous near-infrared spectroscopy and functional magnetic resonance imaging.
A high temporal correlation between the BOLD signal (fMRI) and the deoxy-hemoglobin concentration (NIRS) in the subjects who exhibited low fluctuations in superficial head tissues is found. Expand
Phase fluctuation in phospholipid membranes revealed by Laurdan fluorescence.
This work has used 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) to study phase coexistence and phase interconversion in membrane model systems and introduced the concept of "generalized polarization". Expand
The phasor approach to fluorescence lifetime imaging analysis.
The phasor approach has the potential to simplify the way data are analyzed in FLIM, paving the way for the analysis of large data sets and, in general, making the FLIM technique accessible to the nonexpert in spectroscopy and data analysis. Expand
Visualizing lipid structure and raft domains in living cells with two-photon microscopy
This work directly visualize membrane lipid structure of living cells by using two-photon microscopy and provides evidence that membrane coverage by lipid rafts and their fluidity are principally governed by cholesterol content, thereby providing strong support for the lipid raft hypothesis. Expand
Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope
Raster image correlation spectroscopy (RICS) is a new and novel technique for measuring molecular dynamics and concentrations from fluorescence confocal images. The RICS technique extractsExpand