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The ARP2/3 complex: an actin nucleator comes of age
  • E. Goley, M. Welch
  • Medicine, Biology
  • Nature Reviews Molecular Cell Biology
  • 29 September 2006
The cellular functions of the actin cytoskeleton require precise regulation of both the initiation of actin polymerization and the organization of the resulting filaments. The actin-relatedExpand
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Critical conformational changes in the Arp2/3 complex are induced by nucleotide and nucleation promoting factor.
Actin nucleation and branching by the Arp2/3 complex is tightly regulated by activating factors. However, the mechanism of Arp2/3 complex activation remains unclear. We used fluorescence resonanceExpand
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Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity.
The Arp2/3 complex is a seven-protein assembly that is critical for actin nucleation and branching in cells. Here we report the reconstitution of active human Arp2/3 complex after expression of allExpand
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Dynamic Nuclear Actin Assembly by Arp2/3 Complex and a Baculovirus WASP-Like Protein
Diverse bacterial and viral pathogens induce actin polymerization in the cytoplasm of host cells to facilitate infection. Here, we describe a pathogenic mechanism for promoting dynamic actin assemblyExpand
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Assembly of the Caulobacter cell division machine
Cytokinesis in Gram‐negative bacteria is mediated by a multiprotein machine (the divisome) that invaginates and remodels the inner membrane, peptidoglycan and outer membrane. Understanding the orderExpand
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Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility
The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. In vitro, the actin monomer-binding region of ActA is critical for stimulatingExpand
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Imaging-based identification of a critical regulator of FtsZ protofilament curvature in Caulobacter.
FtsZ is an essential bacterial GTPase that polymerizes at midcell, recruits the division machinery, and may generate constrictive forces necessary for cytokinesis. However, many of the mechanisticExpand
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A Rickettsia WASP‐like protein activates the Arp2/3 complex and mediates actin‐based motility
Spotted fever group Rickettsia are obligate intracellular pathogens that exploit the host cell actin cytoskeleton to promote motility and cell‐to‐cell spread. Although other pathogens such asExpand
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DipM links peptidoglycan remodelling to outer membrane organization in Caulobacter
Cell division in Gram‐negative organisms requires coordinated invagination of the multilayered cell envelope such that each daughter receives an intact inner membrane, peptidoglycan (PG) layer andExpand
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Form and function of the bacterial cytokinetic ring.
Bacterial cytokinesis depends upon the tubulin-like GTPase FtsZ, which polymerizes into an annular structure at midcell (the Z-ring) that defines the division site. The Z-ring nucleates assembly ofExpand
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