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Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels
Eukaryotic cells contain assemblies of RNAs and proteins termed RNA granules. Many proteins within these bodies contain KH or RRM RNA-binding domains as well as low complexity (LC) sequences ofExpand
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How MAP Kinases Are Regulated (*)
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Activation Mechanism of the MAP Kinase ERK2 by Dual Phosphorylation
The structure of the active form of the MAP kinase ERK2 has been solved, phosphorylated on a threonine and a tyrosine residue within the phosphorylation lip. The lip is refolded, bringing theExpand
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Phosphorylation of the MAP Kinase ERK2 Promotes Its Homodimerization and Nuclear Translocation
The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. We find thatExpand
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Yersinia YopJ Acetylates and Inhibits Kinase Activation by Blocking Phosphorylation
Yersinia species use a variety of type III effector proteins to target eukaryotic signaling systems. The effector YopJ inhibits mitogen-activated protein kinase (MAPK) and the nuclear factor κBExpand
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Atomic structure of the MAP kinase ERK2 at 2.3 Å resolution
The structure of the MAP kinase ERK2, a ubiquitous protein kinase target for regulation by Ras and Raf, has been solved in its unphosphorylated low-activity conformation to a resolution of 2.3 A. TheExpand
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WNK1, a Novel Mammalian Serine/Threonine Protein Kinase Lacking the Catalytic Lysine in Subdomain II*
We have cloned and characterized a novel mammalian serine/threonine protein kinase WNK1 (withno lysine (K)) from a rat brain cDNA library. WNK1 has 2126 amino acids and can be detected as a proteinExpand
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Crystal structures of MAP kinase p38 complexed to the docking sites on its nuclear substrate MEF2A and activator MKK3b.
The structures of the MAP kinase p38 in complex with docking site peptides containing a phi(A)-X-phi(B) motif, derived from substrate MEF2A and activating enzyme MKK3b, have been solved. The peptidesExpand
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Regulation of WNK1 by an Autoinhibitory Domain and Autophosphorylation*
WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. These enzymes have been linked to a genetically definedExpand
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Chloride Sensing by WNK1 Involves Inhibition of Autophosphorylation
Structural analysis reveals how chloride inhibits the activation of a kinase implicated in hypertension. Inhibited by Chloride in the Active Site Chloride ions are essential for regulation of bloodExpand
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