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An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications
- Sophie Champlot, Camille Berthelot, M. Pruvost, E. A. Bennett, T. Grange, E. Geigl
- BiologyPloS one
- 28 September 2010
A versatile multistrategy decontamination procedure for PCR reagents is developed that allows efficient reagent decontamination while preserving the efficiency of PCR amplification of minute quantities of DNA.
Past climate changes, population dynamics and the origin of Bison in Europe
The paleogenetic analysis of bison remains from the last 50,000 years reveals the influence of climate changes on the dynamics of the various bison populations in Europe, only one of which survived into the Holocene, where it experienced severe reductions in its genetic diversity.
The palaeogenetics of cat dispersal in the ancient world
Ancient DNA analysis of archaeological cat remains shows cats dispersed along trade routes from the Neolithic era onwards, while its gene pool shows admixture from multiple geographical sources and…
Early bone diagenesis in temperate environments: Part I: Surface features and histology
Library construction for ancient genomics: single strand or double strand?
- E. A. Bennett, Diyendo Massilani, G. Lizzo, J. Daligault, E. Geigl, T. Grange
- 1 June 2014
It is shown that the single-stranded library construction method improves the relative recovery of endogenous to exogenous DNA for most, but not all, of the ancient extracts.
Freshly excavated fossil bones are best for amplification of ancient DNA
- M. Pruvost, R. Schwarz, E. Geigl
- Geography, Environmental ScienceProceedings of the National Academy of Sciences
- 16 January 2007
It is demonstrated that freshly excavated and nontreated unwashed bones contain six times more DNA and yield twice as many authentic DNA sequences as bones treated with standard procedures, and calls for a revision of the postexcavation treatment of fossil bones to better preserve the genetic heritage of past life forms.
Genomic distribution and transcription of solitary HERV-K LTRs.
The human genome contains a family of endogenous retroviruses, HERV-K, with sequence homology to the B-type mouse mammary tumor virus, which entered the primate genome about 33-40 million years ago and is estimated to contain about 25,000 copies of HERV -related LTRs, which are distributed over most human chromosomes in an irregular manner.
Ancient genomes from present-day France unveil 7,000 years of its demographic history
- Samantha Brunel, E. A. Bennett, M. Pruvost
- Biology, HistoryProceedings of the National Academy of Sciences
- 26 May 2020
The population structure from the Mesolithic to the Iron Age in France is shown and the changing frequency of genotypes associated with phenotypic traits is traced, showing the presence of Magdalenian-associated ancestry beyond the Iberian Peninsula in the Late Paleolithic.
A New High-Throughput Approach to Genotype Ancient Human Gastrointestinal Parasites
The study of twenty-five 100 to 7,200 year-old archeological samples proved a PCR-based approach coupled with next-generation sequencing to perform precise taxonomic identification of parasitic helminths directly from archeological sediments to be a powerful, reliable and efficient approach for species determination.
Phylogeography, genetic structure and population divergence time of cheetahs in Africa and Asia: evidence for long-term geographic isolates
It is demonstrated that these extant Iranian cheetahs are an autochthonous monophyletic population and the last representatives of the Asiatic subspecies A. j.