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Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system.
Thyroid hormone receptor orthologues from invertebrate species with emphasis on Schistosoma mansoni
Invertebrate TR genes originated from a common ancestor of the Bilateria, and exhibited the ability to form a dimer with RXR prior to the emergence of the vertebrate TRs and were able to bind to vertebrates TR core DNA elements as a monomer or homodimer.
A poxvirus-encoded uracil DNA glycosylase is essential for virus viability
Attempts to inactivate the HindIII D4R gene of vaccinia virus by targeted insertion of a dominant xanthine-guanine phosphoribosyltransferase selection marker or direct inserted of a frame-shifted oligonucleotide were uniformly unsuccessful demonstrating that the poxvirus enzyme is essential for virus viability.
Domain Structure of the Vaccinia Virus mRNA Capping Enzyme
D1R represents a functionally independent domain of the mRNA capping enzyme, fully competent in substrate binding and catalysis at both the triphosphatase and guanylyltransferase active sites, and is found to be kinetically equivalent to the holoenzyme.
The mRNA (guanine-7-)methyltransferase domain of the vaccinia virus mRNA capping enzyme. Expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme.
A low, but reproducible, level of mRNA (guanine-7-)methyltransferase activity was detected in the isolated D1R498-844 subunit demonstrating that the active site resides solely within the large subunit of the capping enzyme.
Vaccinia virus gene D8 encodes a virion transmembrane protein
These studies demonstrated that the wild-type D8 protein was a transmembrane protein with a major extraviral domain that was released largely intact from the virus by trypsin, confirming the hypothesis that although it is associated with the virus, it is in a conformation different from that of theWild-type protein.
Schistosoma mansoni male–female interactions
The role of the sexes in mate attraction, mate choice, mating behavior, female growth, female reproductive development, egg production, and other sex-evolved functions are studied.
Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment.
The nucleotide sequence of the 16,059-bp HindIII D fragment from vaccinia virus strain WR is determined, which reveals a set of 22 open reading frames (ORFs), which are capable of encoding proteins ranging from 61 to 844 amino acids in length.
Characterization of the vaccinia virus RNA 5'-triphosphatase and nucleotide triphosphate phosphohydrolase activities. Demonstrate that both activities are carried out at the same active site.
D1R1-545, an active subdomain of the large subunit of vaccinia virus mRNA capping enzyme possessing ATPase, RNA 5'-triphosphatase, and guanylyltransferase activities, was expressed in Escherichia
Evolution of a novel subfamily of nuclear receptors with members that each contain two DNA binding domains
3 nuclear receptors named 2DBD-NRs, were identified from the flatworm Schistosoma mansoni that each possess a novel set of two DBDs in tandem with a LBD, demonstrating that certain NR gene underwent recent duplication in Prostostome lineages after the split of the Prostosteromia and Deuterostomia.