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Molecular Cloning: A Laboratory Manual
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
An immunogenic personal neoantigen vaccine for patients with melanoma
The feasibility, safety, and immunogenicity of a vaccine that targets up to 20 predicted personal tumour neoantigens is demonstrated and a strong rationale for further development of this approach, alone and in combination with checkpoint blockade or other immunotherapies is provided.
Isolation and characterization of genomic and cDNA clones of human erythropoietin
The cloning of the human erythropoietin gene and the expression of an erythrocytes cDNA clone in a transient mammalian expression system to yield a secreted product with biological activity are described.
Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trial
It is demonstrated that a strategy that uses multi-epitope, personalized neoantigen vaccination, which has previously been tested in patients with high-risk melanoma, is feasible for tumours such as glioblastoma, which typically have a relatively low mutation load and an immunologically ‘cold’ tumour microenvironment.
Characterisation of deletions which affect the expression of fetal globin genes in man
Correlation of haematological data and the location of deletions in two cases of HPFH and one case of δβ-thalassaemia suggest that a region of DNA located near the 5′-end of the δ- globin gene may be involved in the suppression in cis of γ-globin gene expression in adults.
Immunotherapy advances for glioblastoma.
Preliminary results of clinical studies demonstrate that varied vaccine strategies have achieved encouraging evidence of clinical benefit for glioblastoma patients, although multiple variables will likely require systematic investigation before optimal outcomes are realized.
A secreted PD-L1 splice variant that covalently dimerizes and mediates immunosuppression
- K. Mahoney, S. Shukla, +14 authors G. Freeman
- Medicine, ChemistryCancer Immunology, Immunotherapy
- 18 December 2018
This is the first report of a form of secreted PD-L1 that homodimerizes and is functionally active and may function as a paracrine negative immune regulator within the tumor, since secPD-L 1 does not require a cell-to-cell interaction to mediate its inhibitory effect.
Getting Personal with Neoantigen-Based Therapeutic Cancer Vaccines
- N. Hacohen, E. Fritsch, T. Carter, E. Lander, Catherine J. Wu
- MedicineCancer Immunology Research
- 7 April 2013
Although many challenges remain in producing and testing neoantigen-based vaccines customized for each patient, a neoantige vaccine offers a promising new approach to induce highly focused antitumor T cells aimed at eradicating cancer cells.
Systematic identification of personal tumor-specific neoantigens in chronic lymphocytic leukemia.
A streamlined approach for the prediction and validation of neoantigens derived from individual tumors and presented by patient-specific HLA alleles is implemented, suggesting that neoantIGens are frequent in most tumors.
The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
- K. Jacobs, R. Rudersdorf, S. D. Neill, J. Dougherty, E. L. Brown, E. Fritsch
- Biology, MedicineNucleic acids research
- 25 May 1988
The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.