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Effects of snowdrop lectin (GNA) delivered via artificial diet and transgenic plants on the development of tomato moth (Lacanobia oleracea) larvae in laboratory and glasshouse trials.
TLDR
The assays show that the insecticidal effects of GNA can be observed both in vitro when fed in artificial diet and in planta, and can be demonstrated in the glasshouse as well as under growth cabinet conditions. Expand
Biochemical characterization of midgut digestive proteases from Mamestra brassicae (cabbage moth; Lepidoptera: Noctuidae) and effect of soybean Kunitz inhibitor (SKTI) in feeding assays.
TLDR
Qualitative and quantitative changes in proteinase activity bands after gel electrophoresis of gut extracts were evident in SKTI-fed larvae when compared with controls, with increases in levels of most bands, appearance of new bands, and a decrease in the major proteinase band present in extracts from control insects. Expand
In vitro and in vivo binding of snowdrop (Galanthus nivalis agglutinin; GNA) and jackbean (Canavalia ensiformis; Con A) lectins within tomato moth (Lacanobia oleracea) larvae; mechanisms of
TLDR
Results suggested that both lectins are able to exert systemic effects via transport from the gut contents to the haemolymph across the gut epithelium and to contribute to the higher levels of toxicity shown by Con A, relative to GNA, in previous long term bioassays. Expand
Exploring the chemical safety of fly larvae as a source of protein for animal feed
TLDR
Safety data is presented from the larvae of the four fly species that have perhaps the greatest economic relevance in relation to their use as animal feed being: house fly, blue bottle, red bottle, blow fly and black soldier fly. Expand
Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) and the rice brown planthopper, Nilaparvata lugens (Hemiptera:
TLDR
The SFI1/GNA fusion protein, comprising of snowdrop lectin fused to an insecticidal spider venom neurotoxin, was tested for toxicity against the rice brown planthopper Nilaparvata lugens and the peach-potato aphid Myzus persicae by incorporation into artificial diets. Expand
Fusion to Snowdrop Lectin Magnifies the Oral Activity of Insecticidal ω-Hexatoxin-Hv1a Peptide by Enabling Its Delivery to the Central Nervous System
TLDR
In addition to mediating transport of HV1a across the gut epithelium in lepidopteran larvae, GNA is also capable of delivering Hv1a to sites of action within the insect central nervous system. Expand
Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion.
TLDR
SFI1/GNA and similar fusion proteins offer a novel and effective approach for delivering haemolymph active toxins by oral administration, which could be used in crop protection by expression in transgenic plants. Expand
Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion.
TLDR
GNA/Manse-AS and similar fusion proteins offer a novel and effective strategy for delivering insect neuropeptides by oral administration, which could be used in conjunction with expression in transgenic plants to give crop protection in the field. Expand
Sugar transporters of the major facilitator superfamily in aphids; from gene prediction to functional characterization
TLDR
Analysis of the pea aphid genome using signatures specific to the Major Facilitator Superfamily and the Sugar_tr family has identified 54 genes encoding potential sugar transporters, of which 38 have corresponding ESTs. Expand
A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects
TLDR
The ButaIT/GNA recombinant fusion protein is toxic to lepidopteran larvae both when injected and when fed orally, showing the utility of GNA as a carrier to transport potentially toxic peptides and proteins across the insect gut. Expand
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