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Analysis of repetitive element DNA methylation by MethyLight
TLDR
This work reports the development of quantitative MethyLight assays to determine the levels of methylated and unmethylated repeats, namely, Alu and LINE-1 sequences and the centromeric satellite alpha (Satα) and juxtacentromeric satellites 2 (Sat2) DNA sequences.
Differential effects of CYP2E1 status on the metabolic activation of the colon carcinogens azoxymethane and methylazoxymethanol.
TLDR
It is suggested that agents that modify CYP2E1 activity at the tumor initiation stage might either enhance or inhibit colon carcinogenesis, depending on whether AOM or MAMAc is used as the carcinogen.
Oxidative DNA and RNA damage in the livers of Sprague-Dawley rats treated with the hepatocarcinogen 2-nitropropane.
TLDR
These findings are consistent with a mechanism of hepatocarcinogenicity of 2-NP based on damage to nucleic acids from the intracellular generation of reactive forms of oxygen and/or the 2- NP free radical.
DNA hypomethylation and unusual chromosome instability in cell lines fromICF syndrome patients
TLDR
The results suggest that decondensation of 1qh and 16qh often leads to unresolved Holliday junctions, chromosome breakage, arm missegregation, and the formation of multiradials that may yield more stable chromosomal abnormalities, such as translocations.
Quantitative analysis of associations between DNA hypermethylation, hypomethylation, and DNMT RNA levels in ovarian tumors
TLDR
It is suggested that there is not a simple association of gene hypermethylation in cancer with altered DNMT RNA levels, and that this hyper methylation is neither the result nor the cause of satellite and global DNA hypomethylation.
Effects of green and black tea on hepatic xenobiotic metabolizing systems in the male F344 rat.
TLDR
Beverages widely used by man altered host biochemistry as regards specific phase I and II enzymes in liver of rat and specific serum parameters were studied.
Hypomethylation and hypermethylation of DNA in Wilms tumors
TLDR
The overall genomic hypomethylation frequently observed in cancers is probably not just a response or a prelude to hypermethylation elsewhere in the genome, which suggests that the DNA hypometHylation contributes independently to oncogenesis or tumor progression.
Investigations into the metabolism and mode of action of the colon carcinogens 1,2‐dimethylhydrazine and azoxymethane
  • E. Fiala
  • Chemistry, Biology
    Cancer
  • 1 November 1977
TLDR
On‐going studies with AOM‐14C indicate that in male F‐344 rats, this carcinogen is rapidly metabolized to 14CO2 and to methylazoxymethanol‐ 14C (MAM), which, along with other metabolites, appears in the urine.
Metabolism of azoxymethane, methylazoxymethanol and N-nitrosodimethylamine by cytochrome P450IIE1.
TLDR
Results provide conclusive evidence that AOM, MAM and NDMA are metabolized by the same form of rat liver cytochrome P450, and the stoichiometry of NDMA products formed in these reactions indicates that denitrosation, a presumed detoxication process, and alpha-hydroxylation, an activation reaction, are also catalyzed by thesame cyto chrome P450 isozyme.
Enhancement of rat liver microsomal metabolism of azoxymethane to methylazoxymethanol by chronic ethanol administration: similarity to the microsomal metabolism of N-nitrosodimethylamine.
TLDR
High-performance liquid chromatographic analysis of the products of the microsomal metabolism of AOM showed that methylazoxymethanol was the only primary metabolite, and Hepatocytes isolated from ethanol-fed rats showed a significantly enhanced sensitivity to AOM- as well as to NDMA-induced unscheduled DNA synthesis, indicating that the increased rate of microsome metabolism induced by ethanol is associated with enhanced carcinogen activation in vitro.
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