• Publications
  • Influence
Role of ATP and glycogen reserves in both paracetamol sulfation and glucuronidation by cultured precision-cut rat liver slices.
TLDR
It is suggested that loss of glycogen impair glucuronide conjugation by decreasing the availability of UDPGA, and that low values of ATP are largely enough to support sulfotransferase activity. Expand
Effects of bacterial endotoxin (lipopolysaccharides) on survival and metabolism of cultured precision-cut rat liver slices.
TLDR
The in vitro model of PCLS reproduces the inhibitory effect of LPS on a CYP3A-dependent activity, allowing a mechanistic approach to study cell-cell interactions. Expand
Role of temperature on protein and mRNA cytochrome P450 3A (CYP3A) isozymes expression and midazolam oxidation by cultured rat precision-cut liver slices.
TLDR
CYP3A1 protein is constitutive and largely expressed in rat liver slices, and regioselective midazolam oxidation appears to be mainly CYP3A2 dependent, which suggests that the loss of CYP 3A2 protein at 37 degrees might be due to a selective targeting leading to proteolytic disposal by the proteasome. Expand
Reoxygenation after cold hypoxic storage of cultured precision-cut rat liver slices: effects on cellular metabolism and drug biotransformation.
TLDR
The results suggest that CYP3A2 did not play a major role in midazolam oxidation and a minor role of non-parenchymal cells is suggested as mediators for deleterious effects developed during the cold storage. Expand
Protective effect of fructose on survival and metabolic capacities of hepatocytes kept overnight under cold hypoxia before normothermic reoxygenation.
TLDR
The protective effect of fructose with regard to hypoxia-induced cell injury in overnight cold preserved hepatocytes was investigated and the cellular metabolic capacities such as protein synthesis and gluconeogenesis from lactate recovered faster during reoxygenation of previously hypoxic fructose- treated cells compared with both control and adenosine-treated cells. Expand
Effect of cold hypoxic storage and reoxygenation at 37°C of cultured precision-cut rat liver slices on paracetamol metabolism
TLDR
The maintenance of energetic stores during cold preservation appears, therefore, to be a critical issue for the survival and metabolic activity of cells. Expand
...
1
2
...