Secreted production of a custom-designed, highly hydrophilic gelatin in Pichia pastoris.
- M. Werten, W. Wisselink, T. Jansen-van den Bosch, E. De Bruin, F. D. de Wolf
- BiologyProtein Engineering
- 1 June 2001
Despite the fact that gelatins are highly susceptible to proteolysis because of their unfolded structure, the recombinant protein was shown to be fully intact by SDS-PAGE, N-terminal sequencing, gel filtration chromatography and mass spectrometry.
Endogenous prolyl 4-hydroxylation in Hansenula polymorpha and its use for the production of hydroxylated recombinant gelatin.
It is found that the well-known protein expression host Hansenula polymorpha unexpectedly does have the endogenous capacity for prolyl 4-hydroxylation.
Recombinant human C1-inhibitor produced in Pichia pastoris has the same inhibitory capacity as plasma C1-inhibitor.
Will there be blood? Explaining violence during coups d’état
- E. De Bruin
- Political ScienceJournal of Peace Research
- 6 June 2019
Although just under half of all coup d’état attempts involve fatalities, there has been surprisingly little attention to the conditions under which coups turn violent. Existing research emphasizes…
Mapping coercive institutions: The State Security Forces dataset, 1960–2010
- E. De Bruin
- Computer Science, Political Science
- 26 May 2020
The SSF dataset is presented, which includes 375 security forces in 110 countries, 1960–2010, and shows how it can be used to deepen the understanding of coup-proofing and strategic substitution and identifies additional research uses of the dataset.
Pharmaceutical proteins from methylotrophic yeasts.
Forum: A Coup At the Capitol? Conceptualizing Coups and Other Antidemocratic Actions
- Jonathan M Powell, Salah Ben Hammou, E. De Bruin
- Political ScienceInternational Studies Review
- 11 February 2022
The term “coup” has been used to describe a diverse range of events. Although recent decades have seen the academic study of coups focus on an increasingly narrow type of military intervention in…
Production, purification and characterisation of recombinant Fahsin, a novel antistasin-type proteinase inhibitor.
Militarized Policing in the Middle East and North Africa
- E. De Bruin, Zachary Karabatak
- Political ScienceThe Journal of the Middle East and Africa
- 27 December 2021
ABSTRACT This article explains changing patterns in police militarization in the Middle East and North Africa. It presents new data on police forces in nineteen countries in the region, 1946–2020,…
Counterbalancing and Coups d’État
- E. De Bruin
- Political Science
- 19 November 2020
Counterbalancing is a coup-proofing strategy in which rulers divide the state’s coercive power among multiple, overlapping security forces to hedge against defection from the regular military. There…