Proliferation, maturation and germination of Castanea sativa Mill. Somatic embryos originated from leaf explants.
- E. Corredoira, A. Ballester, A. Viéitez
- Medicine, BiologyAnnals of Botany
- 1 July 2003
Experiments were performed to determine the influence of proliferation medium on the maintenance of embryogenic competence and on repetitive embryogenesis in Castanea sativa Mill and the best results were obtained with 3 % maltose-matured somatic embryos, giving rise to 6 % plant recovery.
Thidiazuron-induced high-frequency plant regeneration from leaf explants of Paulownia tomentosa mature trees
- E. Corredoira, A. Ballester, A. Vieitez
- BiologyPlant Cell Tissue and Organ Culture
- 1 August 2008
Attempts were made to study the effect of thidiazuron (TDZ) on adventitious shoot induction and plant development in Paulownia tomentosa explants derived from mature trees, with results indicating that shoot formation capacity was influenced by the genotype.
Application of biotechnological tools to Quercus improvement
- A. Vieitez, E. Corredoira, A. Ballester
- Environmental ScienceEuropean Journal of Forest Research
- 1 May 2012
This review provides a summary of the advances made in the application of biotechnological tools to specific oak species and suggests that efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future to improve the practical application of clonal forestry in recalcitrant species such as oaks.
In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra
- A. Viéitez, E. Corredoira, A. Ballester, F. Munoz, J. Durán, M. Ibarra
- BiologyPlant Cell Tissue and Organ Culture
- 22 May 2009
North American oak species, with their characteristic strong episodic seasonal shoot growth, are highly problematic for clonal micropropagation, resulting in the inability to achieve a stabilized shoot multiplication stage, so an initial 5-day dark period generally improved the rooting response, but was detrimental to the quality of regenerated plantlets.
Cryopreservation of zygotic embryo axes and somatic embryos of European chestnut.
- E. Corredoira, M. C. San-José, A. Ballester, A. Viéitez
- Biology, MedicineCryo-letters
- 2004
Experiments demonstrating the feasibility of long-term conservation of Castanea sativa germplasm through cryopreservation of embryonic axes or somatic embryo clumps show that desiccation to moisture contents less than 19 % produced damage resulting in loss of organized plant development after cryostorage.
GENETIC TRANSFORMATION OF CASTANEA SATIVA MILL. BY AGROBACTERIUM TUMEFACIENS
- E. Corredoira, M. C. San-José, A. Ballester, A. Viéitez
- Biology
- 1 October 2005
Trabajo presentado en el III International Chestnut Congress, celebrado en Chaves (Portugal) en octubre de 2004
Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees
- M. C. San-José, E. Corredoira, A. Viéitez
- Biology, MedicinePlant Cell Reports
- 8 April 2010
Histological study confirmed an indirect somatic embryogenesis process from shoot tip explants, in which leaf primordia and the corresponding axial zones were involved in generating callus, whereas the apical meristem itself did not proliferate.
Non-Zygotic Embryogenesis in Hardwood Species
- E. Corredoira, S. Merkle, A. Viéitez
- BiologyCritical reviews in plant sciences
- 2 January 2019
Main concerns associated with the application of non-zygotic or somatic embryogenesis for large-scale propagation of elite hardwoods are related to the embryo maturation, germination, and plantlet conversion steps, and these are highlighted in this review.
Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.
- P. van Dillewijn, J. L. Couselo, J. Ramos
- EngineeringEnvironmental Science and Technology
- 1 October 2008
Transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers.
Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
- E. Corredoira, M. C. San-José, A. Viéitez, A. Ballester
- BiologyPlant Cell Tissue and Organ Culture
- 2 October 2007
It was found that the use of somatic embryos isolated in the globular stage or clumps of 2–3 embryos in globular/heart-shaped stages was more effective than when embryos at the cotyledonary stage were used.
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