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The isolation, culture and regeneration of Petunia leaf protoplasts.
Protoplasts isolated from varieties of P. hybrida were found to differ in their cultural requirements, and cell colonies derived from individual mesophyll protoplasts grow rapidly upon subculture, to produce callus capable of shoot differentiation and ultimately whole plant formation. Expand
Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose
Protoplasts were readily isolated from cell suspension of four rice cultivars, including japonica and indica types, when maintained in an amino acid-based culture medium and produced distinct embryo-like structures. Expand
The determination of amino-acids with ninhydrin
A systematic review of the mechanism and application of the ninhydrin colour test for amino-acids, with a note on its use in the identification of protein hydrolysates. -- AATA
A Method for the Isolation of Plant Protoplasts and Vacuoles
Root tips of tomato seedlings were selected for investigation, and a fungal cellulase obtained from Myrothecium verrucaria4 (kindly supplied by Dr. R. Whitaker) was employed. Expand
Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene
Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 107 following PEG-induced uptake of Sma1 linearised pABD1 plasmid, and tissues maintained on this level of antibiotic expressed APH(3′)II activity, and contained DNA that hybridised to a probe with the APH (3′),II gene. Expand
Efficient Plant Regeneration from Rice Protoplasts Through Somatic Embryogenesis
It is reported that plant regeneration can be achieved efficiently and reproducibly from protoplasts of rice isolated from cell suspension cultures. Expand
Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus
Whether these novel inoculations with G. diazotrophicus are likely to enable non-nodulating endophytic nitrogen fixation and whether these inoculations can also provide a plant system to investigate the endosymbiotic theory of the origin of eukaryotic organelles is discussed. Expand
Interactions of rhizobia with rice and wheat
Naringenin does not appear to be acting as a carbon source and may act as a signal molecule for intercellular colonization of rice and wheat by ORS571 by a mechanism which is nod gene-independent, unlike nodule formation in Sesbania rostrata. Expand